Long-range organization of bacteriochlorophyll in chlorosomes of Chlorobium tepidum investigated by cryo-electron microscopy

Gert T. Oostergetel, Michael Reus, Aline Gomez Maqueo Chew, Donald A. Bryant, Egbert J. Boekema, Alfred R. Holzwarth

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

Intact chlorosomes of Chlorobium tepidum were embedded in amorphous ice layers and examined by cryo-electron microscopy to study the long-range organization of bacteriochlorophyll (BChl) layers. End-on views reveal that chlorosomes are composed of several multi-layer tubules of variable diameter (20-30 nm) with some locally undulating non-tubular lamellae in between. The multi-layered tubular structures are more regular and larger in a C. tepidum mutant that only synthesizes [8-ethyl, 12-methyl]-BChl d. Our data show that wild-type C. tepidum chlorosomes do not have a highly regular, long-range BChl c layer organization and that they contain several multi-layered tubules rather than single-layer tubules or exclusively undulating lamellae as previously proposed.

Original languageEnglish (US)
Pages (from-to)5435-5439
Number of pages5
JournalFEBS Letters
Volume581
Issue number28
DOIs
StatePublished - Nov 27 2007

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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