Long-Term Organic Nutrient Management Fosters the Eubacterial Community Diversity in the Indian Semi-arid Alfisol as Revealed by Length Heterogeneity-PCR

Dananjeyan Balachandar, Melissa S. Doud, Lisa Schneper, De Etta Mills, Kalai Mathee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Agricultural practices influence the community structure and functional diversity of soil microorganisms. In the present study, the impact of nutrient-management systems on the changes in the biological properties of Indian semi-arid Alfisol was assessed. The long-term organically managed (OGF) and inorganically fertilized (IGF) soils from century-old experimental plots were compared for eubacterial diversity using amplicon length heterogeneity PCR (LH-PCR) targeting three hypervariable domains (V1, V1_V2, and V3) of 16S rRNA gene. Of these domains, V1_V2 could discriminate the bacterial communities between the soil types. The relative ratios of amplicons differed between OGF and ICF soils, and eubacterial diversity was decreased substantially because of the inorganic chemical fertilizers, as compared to organic amendments. The Bray-Curtis similarity index and diversity indices of amplicons were greater in OGF soil than in ICF soil. This polyphasic approach revealed that the diversity and functionality of the soil eubacterial community were encouraged by long-term organic manures more than inorganic chemical fertilizers.

Original languageEnglish (US)
Pages (from-to)189-203
Number of pages15
JournalCommunications in Soil Science and Plant Analysis
Volume45
Issue number2
DOIs
StatePublished - Jan 1 2014

Fingerprint

Alfisol
Alfisols
nutrient management
nutrient
inorganic compounds
soil
fertilizers
similarity index
soil microorganism
functional diversity
agricultural practice
soil microorganisms
diversity index
bacterial communities
targeting
management systems
soil type
manure
soil types
community structure

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Soil Science

Cite this

Balachandar, Dananjeyan ; Doud, Melissa S. ; Schneper, Lisa ; Mills, De Etta ; Mathee, Kalai. / Long-Term Organic Nutrient Management Fosters the Eubacterial Community Diversity in the Indian Semi-arid Alfisol as Revealed by Length Heterogeneity-PCR. In: Communications in Soil Science and Plant Analysis. 2014 ; Vol. 45, No. 2. pp. 189-203.
@article{a02843feda2f42979a9e12123860d917,
title = "Long-Term Organic Nutrient Management Fosters the Eubacterial Community Diversity in the Indian Semi-arid Alfisol as Revealed by Length Heterogeneity-PCR",
abstract = "Agricultural practices influence the community structure and functional diversity of soil microorganisms. In the present study, the impact of nutrient-management systems on the changes in the biological properties of Indian semi-arid Alfisol was assessed. The long-term organically managed (OGF) and inorganically fertilized (IGF) soils from century-old experimental plots were compared for eubacterial diversity using amplicon length heterogeneity PCR (LH-PCR) targeting three hypervariable domains (V1, V1_V2, and V3) of 16S rRNA gene. Of these domains, V1_V2 could discriminate the bacterial communities between the soil types. The relative ratios of amplicons differed between OGF and ICF soils, and eubacterial diversity was decreased substantially because of the inorganic chemical fertilizers, as compared to organic amendments. The Bray-Curtis similarity index and diversity indices of amplicons were greater in OGF soil than in ICF soil. This polyphasic approach revealed that the diversity and functionality of the soil eubacterial community were encouraged by long-term organic manures more than inorganic chemical fertilizers.",
author = "Dananjeyan Balachandar and Doud, {Melissa S.} and Lisa Schneper and Mills, {De Etta} and Kalai Mathee",
year = "2014",
month = "1",
day = "1",
doi = "10.1080/00103624.2013.841919",
language = "English (US)",
volume = "45",
pages = "189--203",
journal = "Communications in Soil Science and Plant Analysis",
issn = "0010-3624",
publisher = "Taylor and Francis Ltd.",
number = "2",

}

Long-Term Organic Nutrient Management Fosters the Eubacterial Community Diversity in the Indian Semi-arid Alfisol as Revealed by Length Heterogeneity-PCR. / Balachandar, Dananjeyan; Doud, Melissa S.; Schneper, Lisa; Mills, De Etta; Mathee, Kalai.

In: Communications in Soil Science and Plant Analysis, Vol. 45, No. 2, 01.01.2014, p. 189-203.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Long-Term Organic Nutrient Management Fosters the Eubacterial Community Diversity in the Indian Semi-arid Alfisol as Revealed by Length Heterogeneity-PCR

AU - Balachandar, Dananjeyan

AU - Doud, Melissa S.

AU - Schneper, Lisa

AU - Mills, De Etta

AU - Mathee, Kalai

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Agricultural practices influence the community structure and functional diversity of soil microorganisms. In the present study, the impact of nutrient-management systems on the changes in the biological properties of Indian semi-arid Alfisol was assessed. The long-term organically managed (OGF) and inorganically fertilized (IGF) soils from century-old experimental plots were compared for eubacterial diversity using amplicon length heterogeneity PCR (LH-PCR) targeting three hypervariable domains (V1, V1_V2, and V3) of 16S rRNA gene. Of these domains, V1_V2 could discriminate the bacterial communities between the soil types. The relative ratios of amplicons differed between OGF and ICF soils, and eubacterial diversity was decreased substantially because of the inorganic chemical fertilizers, as compared to organic amendments. The Bray-Curtis similarity index and diversity indices of amplicons were greater in OGF soil than in ICF soil. This polyphasic approach revealed that the diversity and functionality of the soil eubacterial community were encouraged by long-term organic manures more than inorganic chemical fertilizers.

AB - Agricultural practices influence the community structure and functional diversity of soil microorganisms. In the present study, the impact of nutrient-management systems on the changes in the biological properties of Indian semi-arid Alfisol was assessed. The long-term organically managed (OGF) and inorganically fertilized (IGF) soils from century-old experimental plots were compared for eubacterial diversity using amplicon length heterogeneity PCR (LH-PCR) targeting three hypervariable domains (V1, V1_V2, and V3) of 16S rRNA gene. Of these domains, V1_V2 could discriminate the bacterial communities between the soil types. The relative ratios of amplicons differed between OGF and ICF soils, and eubacterial diversity was decreased substantially because of the inorganic chemical fertilizers, as compared to organic amendments. The Bray-Curtis similarity index and diversity indices of amplicons were greater in OGF soil than in ICF soil. This polyphasic approach revealed that the diversity and functionality of the soil eubacterial community were encouraged by long-term organic manures more than inorganic chemical fertilizers.

UR - http://www.scopus.com/inward/record.url?scp=84891501467&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891501467&partnerID=8YFLogxK

U2 - 10.1080/00103624.2013.841919

DO - 10.1080/00103624.2013.841919

M3 - Article

AN - SCOPUS:84891501467

VL - 45

SP - 189

EP - 203

JO - Communications in Soil Science and Plant Analysis

JF - Communications in Soil Science and Plant Analysis

SN - 0010-3624

IS - 2

ER -