Long term persistence of cytomegalovirus genome in cultured human cells of prostatic origin

F. Rapp, L. Geder, D. Murasko, R. Lausch, Roger Ladda, E. S. Huang, M. M. Webber

Research output: Contribution to journalArticle

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Abstract

Cells from prostatic tissue obtained from a 3 yr old male donor exhibited scattered foci of cytopathology on primary culture. A virus was isolated in its shown by serological analysis to be cytomegalovirus (CMV). After a number of cell culture passages, a cell line (designated CMV-Mj-P) was obtained in which foci of infection could no longer be demonstrated, nor could virus be rescued. On continued passage the doubling time of the cells decreased markedly, and the fibroblastoid cells ceased to demonstrate contact inhibition. CMV specific antigen(s) was detected on the surface of the cells by indirect immunofluorescence techniques after exposure of the cultures to iododeoxyuridine. Microcytotoxicity tests established that CMV-Mj-P cells, but not control human prostate cells or human embryonic lung cells, share a membrane antigen with hamster cells transformed by CMV. Nucleic acid hybridization studies revealed that virus genetic information was carried by the human prostate cells and that the cells contained an average of about 10 to 15 genome equivalents of CMV DNA. Karyotypic analysis confirmed that the CMV-Mj-P cells were of human male origin. These results indicate that the cells either have been transformed by CMV or are chronically infected with CMV and releasing virus at levels below detection.

Original languageEnglish (US)
Pages (from-to)982-990
Number of pages9
JournalJournal of Virology
Volume16
Issue number4
StatePublished - Dec 1 1975

Fingerprint

Cytomegalovirus
Cultured Cells
Genome
genome
cells
Viruses
viruses
Prostate
Immunologic Cytotoxicity Tests
Contact Inhibition
Idoxuridine
antigens
Nucleic Acid Hybridization
Antigens
Indirect Fluorescent Antibody Technique
nucleic acid hybridization
hamsters
Cricetinae
fluorescent antibody technique
cell culture

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Rapp, F., Geder, L., Murasko, D., Lausch, R., Ladda, R., Huang, E. S., & Webber, M. M. (1975). Long term persistence of cytomegalovirus genome in cultured human cells of prostatic origin. Journal of Virology, 16(4), 982-990.
Rapp, F. ; Geder, L. ; Murasko, D. ; Lausch, R. ; Ladda, Roger ; Huang, E. S. ; Webber, M. M. / Long term persistence of cytomegalovirus genome in cultured human cells of prostatic origin. In: Journal of Virology. 1975 ; Vol. 16, No. 4. pp. 982-990.
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Rapp, F, Geder, L, Murasko, D, Lausch, R, Ladda, R, Huang, ES & Webber, MM 1975, 'Long term persistence of cytomegalovirus genome in cultured human cells of prostatic origin', Journal of Virology, vol. 16, no. 4, pp. 982-990.

Long term persistence of cytomegalovirus genome in cultured human cells of prostatic origin. / Rapp, F.; Geder, L.; Murasko, D.; Lausch, R.; Ladda, Roger; Huang, E. S.; Webber, M. M.

In: Journal of Virology, Vol. 16, No. 4, 01.12.1975, p. 982-990.

Research output: Contribution to journalArticle

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Rapp F, Geder L, Murasko D, Lausch R, Ladda R, Huang ES et al. Long term persistence of cytomegalovirus genome in cultured human cells of prostatic origin. Journal of Virology. 1975 Dec 1;16(4):982-990.