Longevity in vivo of primary cell wall cellulose synthases

Joseph Lee Hill, Cooper Josephs, William J. Barnes, Charles T. Anderson, Ming Tien

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Key message: Our work focuses on understanding the lifetime and thus stability of the three main cellulose synthase (CESA) proteins involved in primary cell wall synthesis of Arabidopsis. It had long been thought that a major means of CESA regulation was via their rapid degradation. However, our studies here have uncovered that AtCESA proteins are not rapidly degraded. Rather, they persist for an extended time in the plant cell. Abstract: Plant cellulose is synthesized by membrane-embedded cellulose synthase complexes (CSCs). The CSC is composed of cellulose synthases (CESAs), of which three distinct isozymes form the primary cell wall CSC and another set of three isozymes form the secondary cell wall CSC. We determined the stability over time of primary cell wall (PCW) CESAs in Arabidopsis thaliana seedlings, using immunoblotting after inhibiting protein synthesis with cycloheximide treatment. Our work reveals very slow turnover for the Arabidopsis PCW CESAs in vivo. Additionally, we show that the stability of all three CESAs within the PCW CSC is altered by mutations in individual CESAs, elevated temperature, and light conditions. Together, these results suggest that CESA proteins are very stable in vivo, but that their lifetimes can be modulated by intrinsic and environmental cues.

Original languageEnglish (US)
Pages (from-to)279-289
Number of pages11
JournalPlant molecular biology
Volume96
Issue number3
DOIs
StatePublished - Feb 1 2018

Fingerprint

cellulose synthase
Cell Wall
cell walls
Arabidopsis
Isoenzymes
isozymes
Proteins
proteins
Plant Cells
cycloheximide
Cycloheximide
immunoblotting
Seedlings
Immunoblotting
Cellulose
Cues

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Hill, Joseph Lee ; Josephs, Cooper ; Barnes, William J. ; Anderson, Charles T. ; Tien, Ming. / Longevity in vivo of primary cell wall cellulose synthases. In: Plant molecular biology. 2018 ; Vol. 96, No. 3. pp. 279-289.
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Longevity in vivo of primary cell wall cellulose synthases. / Hill, Joseph Lee; Josephs, Cooper; Barnes, William J.; Anderson, Charles T.; Tien, Ming.

In: Plant molecular biology, Vol. 96, No. 3, 01.02.2018, p. 279-289.

Research output: Contribution to journalArticle

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