Longitudinal evaluation of elastase as a marker for the progression of periodontitis.

G. C. Armitage, M. K. Jeffcoat, D. E. Chadwick, E. J. Taggart, Y. Numabe, J. R. Landis, S. L. Weaver, Tonya King

Research output: Contribution to journalArticle

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Abstract

To determine whether elastase levels in gingival crevicular fluid (GCF) could serve as a marker for the progression of periodontitis, we monitored GCF elastase and periodontal status in selected sites in 32 periodontally healthy volunteers and 31 periodontitis patients at intervals over a 6-month period. Clinical measurements included plaque index, gingival index, bleeding on probing, suppuration, probing depth, clinical attachment level, and relative attachment level measured with an automated disk probe. GCF elastase, detected by reaction with a fluorescent substrate, was assessed visually against fluorescence standards and quantitatively with a fluorometer. Bone loss was detected by subtraction radiography of standardized vertical bite-wing radiographs at baseline and 6 months. Mean visual elastase scores (VES) and quantitative elastase measurements were significantly higher (P < 0.001) in sites from periodontitis patients than in sites from healthy volunteers. When bone loss was used as the criterion for disease progression, significantly higher (P < 0.001) visual and quantitative GCF elastase levels were found at progressing sites than in nonprogressing sites in the periodontitis patients. The odds ratios (OR) for the event of developing bone loss with positive 4-minute and 8-minute VES tests were 4.2 (P < 0.001) and 7.4 (P < 0.001), respectively. When corrected for the tendency of progressing sites to be clustered within a subpopulation of patients, the OR for developing bone loss with the 4-minute and 8-minute VES tests were 3.1 (P < 0.007) and 4.9 (P < 0.001), respectively. These data indicate that sites with high levels of elastase are at significantly greater risk for progressive bone loss as assessed by digital subtraction radiography.

Original languageEnglish (US)
Pages (from-to)120-128
Number of pages9
JournalJournal of Periodontology
Volume65
Issue number2
DOIs
StatePublished - Jan 1 1994

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Periodontitis
Pancreatic Elastase
Gingival Crevicular Fluid
Bone and Bones
Periodontal Index
Healthy Volunteers
Odds Ratio
Radiographic Image Enhancement
Suppuration
Bites and Stings
Radiography
Disease Progression
Fluorescence

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Armitage, G. C., Jeffcoat, M. K., Chadwick, D. E., Taggart, E. J., Numabe, Y., Landis, J. R., ... King, T. (1994). Longitudinal evaluation of elastase as a marker for the progression of periodontitis. Journal of Periodontology, 65(2), 120-128. https://doi.org/10.1902/jop.1994.65.2.120
Armitage, G. C. ; Jeffcoat, M. K. ; Chadwick, D. E. ; Taggart, E. J. ; Numabe, Y. ; Landis, J. R. ; Weaver, S. L. ; King, Tonya. / Longitudinal evaluation of elastase as a marker for the progression of periodontitis. In: Journal of Periodontology. 1994 ; Vol. 65, No. 2. pp. 120-128.
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Armitage, GC, Jeffcoat, MK, Chadwick, DE, Taggart, EJ, Numabe, Y, Landis, JR, Weaver, SL & King, T 1994, 'Longitudinal evaluation of elastase as a marker for the progression of periodontitis.', Journal of Periodontology, vol. 65, no. 2, pp. 120-128. https://doi.org/10.1902/jop.1994.65.2.120

Longitudinal evaluation of elastase as a marker for the progression of periodontitis. / Armitage, G. C.; Jeffcoat, M. K.; Chadwick, D. E.; Taggart, E. J.; Numabe, Y.; Landis, J. R.; Weaver, S. L.; King, Tonya.

In: Journal of Periodontology, Vol. 65, No. 2, 01.01.1994, p. 120-128.

Research output: Contribution to journalArticle

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Armitage GC, Jeffcoat MK, Chadwick DE, Taggart EJ, Numabe Y, Landis JR et al. Longitudinal evaluation of elastase as a marker for the progression of periodontitis. Journal of Periodontology. 1994 Jan 1;65(2):120-128. https://doi.org/10.1902/jop.1994.65.2.120