Longitudinal Monitoring of Successive Commercial Layer Flocks for Salmonella enterica Serovar Enteritidis

Thomas N. Denagamage, Paul H. Patterson, Eva A. Wallner-Pendleton, Darrell Trampel, Nikki Shariat, Edward G. Dudley, Bhushan M. Jayarao, Subhashinie Kariyawasam

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Pennsylvania Egg Quality Assurance Program (EQAP) provided the framework for Salmonella Enteritidis (SE) control programs, including the Food and Drug Administration (FDA) mandated Final Egg Rule, for commercial layer facilities throughout the United States. Although flocks with ≥3000 birds must comply with the FDA Final Egg Rule, smaller flocks are exempted from the rule. As a result, eggs produced by small layer flocks may pose a greater public health risk than those from larger flocks. It is also unknown if the EQAPs developed with large flocks in mind are suitable for small- and medium-sized flocks. Therefore, a study was performed to evaluate the effectiveness of best management practices included in EQAPs in reducing SE contamination of small- and medium-sized flocks by longitudinal monitoring of their environment and eggs. A total of 59 medium-sized (3000 to 50,000 birds) and small-sized (<3000 birds) flocks from two major layer production states of the United States were enrolled and monitored for SE by culturing different types of environmental samples and shell eggs for two consecutive flock cycles. Isolated SE was characterized by phage typing, pulsed-field gel electrophoresis (PFGE), and clustered regularly interspaced short palindromic repeats-multi-virulence-locus sequence typing (CRISPR-MVLST). Fifty-four Salmonella isolates belonging to 17 serovars, 22 of which were SE, were isolated from multiple sample types. Typing revealed that SE isolates belonged to three phage types (PTs), three PFGE fingerprint patterns, and three CRISPR-MVLST SE Sequence Types (ESTs). The PT8 and JEGX01.0004 PFGE pattern, the most predominant SE types associated with foodborne illness in the United States, were represented by a majority (91%) of SE. Of the three ESTs observed, 85% SE were typed as EST4. The proportion of SE-positive hen house environment during flock cycle 2 was significantly less than the flock cycle 1, demonstrating that current EQAP practices were effective in reducing SE contamination of medium and small layer flocks.

Original languageEnglish (US)
Pages (from-to)618-625
Number of pages8
JournalFoodborne pathogens and disease
Volume13
Issue number11
DOIs
StatePublished - Nov 1 2016

Fingerprint

Salmonella enteritidis
Salmonella Enteritidis
flocks
monitoring
Ovum
Pulsed Field Gel Electrophoresis
pulsed-field gel electrophoresis
Clustered Regularly Interspaced Short Palindromic Repeats
Birds
Expressed Sequence Tags
United States Food and Drug Administration
egg quality
bacteriophages
Eggs
quality control
Virulence
birds
virulence
Egg Shell
Bacteriophage Typing

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

Cite this

@article{02d2033782f7480db95ba05a6d18c8e5,
title = "Longitudinal Monitoring of Successive Commercial Layer Flocks for Salmonella enterica Serovar Enteritidis",
abstract = "The Pennsylvania Egg Quality Assurance Program (EQAP) provided the framework for Salmonella Enteritidis (SE) control programs, including the Food and Drug Administration (FDA) mandated Final Egg Rule, for commercial layer facilities throughout the United States. Although flocks with ≥3000 birds must comply with the FDA Final Egg Rule, smaller flocks are exempted from the rule. As a result, eggs produced by small layer flocks may pose a greater public health risk than those from larger flocks. It is also unknown if the EQAPs developed with large flocks in mind are suitable for small- and medium-sized flocks. Therefore, a study was performed to evaluate the effectiveness of best management practices included in EQAPs in reducing SE contamination of small- and medium-sized flocks by longitudinal monitoring of their environment and eggs. A total of 59 medium-sized (3000 to 50,000 birds) and small-sized (<3000 birds) flocks from two major layer production states of the United States were enrolled and monitored for SE by culturing different types of environmental samples and shell eggs for two consecutive flock cycles. Isolated SE was characterized by phage typing, pulsed-field gel electrophoresis (PFGE), and clustered regularly interspaced short palindromic repeats-multi-virulence-locus sequence typing (CRISPR-MVLST). Fifty-four Salmonella isolates belonging to 17 serovars, 22 of which were SE, were isolated from multiple sample types. Typing revealed that SE isolates belonged to three phage types (PTs), three PFGE fingerprint patterns, and three CRISPR-MVLST SE Sequence Types (ESTs). The PT8 and JEGX01.0004 PFGE pattern, the most predominant SE types associated with foodborne illness in the United States, were represented by a majority (91{\%}) of SE. Of the three ESTs observed, 85{\%} SE were typed as EST4. The proportion of SE-positive hen house environment during flock cycle 2 was significantly less than the flock cycle 1, demonstrating that current EQAP practices were effective in reducing SE contamination of medium and small layer flocks.",
author = "Denagamage, {Thomas N.} and Patterson, {Paul H.} and Wallner-Pendleton, {Eva A.} and Darrell Trampel and Nikki Shariat and Dudley, {Edward G.} and Jayarao, {Bhushan M.} and Subhashinie Kariyawasam",
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Longitudinal Monitoring of Successive Commercial Layer Flocks for Salmonella enterica Serovar Enteritidis. / Denagamage, Thomas N.; Patterson, Paul H.; Wallner-Pendleton, Eva A.; Trampel, Darrell; Shariat, Nikki; Dudley, Edward G.; Jayarao, Bhushan M.; Kariyawasam, Subhashinie.

In: Foodborne pathogens and disease, Vol. 13, No. 11, 01.11.2016, p. 618-625.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Longitudinal Monitoring of Successive Commercial Layer Flocks for Salmonella enterica Serovar Enteritidis

AU - Denagamage, Thomas N.

AU - Patterson, Paul H.

AU - Wallner-Pendleton, Eva A.

AU - Trampel, Darrell

AU - Shariat, Nikki

AU - Dudley, Edward G.

AU - Jayarao, Bhushan M.

AU - Kariyawasam, Subhashinie

PY - 2016/11/1

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