Loss of Native Flavanols during Fermentation and Roasting Does Not Necessarily Reduce Digestive Enzyme-Inhibiting Bioactivities of Cocoa

Caroline M. Ryan, Weslie Khoo, Liyun Ye, Joshua D. Lambert, Sean F. Okeefe, Andrew P. Neilson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Polyphenol profiles and in vitro digestive enzyme inhibitory activities were compared between cocoa extracts from unfermented beans (UB), fermented beans (FB), unfermented liquor (UL), and fermented liquor (FL). Total polyphenols, total flavanols, and individual flavanols were significantly different between UB/FB and UL/FL. All extracts effectively inhibited α-glucosidase (lowest IC50 = 90.0 μg/mL, UL) and moderately inhibited α-amylase (lowest IC50 = 183 μg/mL, FL) and lipase (lowest IC25 = 65.5 μg/mL, FB). Our data suggest that fermentation does not reduce α-glucosidase inhibition, while roasting may enhance inhibition. For α-amylase, both fermentation and roasting improved inhibition. Finally, for lipase, both fermentation and roasting attenuated inhibition. Conclusive correlations between inhibition and mDP, total polyphenol, and flavanol contents were not found. Our data suggest that enzyme inhibition activities of cocoa are not uniformly reduced by polyphenol/flavanol losses during fermentation and roasting. This paradigm-challenging finding suggests other cocoa constituents, potentially formed during processing, contribute to digestive enzyme inhibition.

Original languageEnglish (US)
Pages (from-to)3616-3625
Number of pages10
JournalJournal of agricultural and food chemistry
Volume64
Issue number18
DOIs
StatePublished - May 11 2016

Fingerprint

Cocoa
Enzyme inhibition
roasting
digestive enzymes
Polyphenols
flavanols
Bioactivity
Fermentation
beans
fermentation
Glucosidases
polyphenols
Enzymes
Amylases
Lipase
glucosidases
enzyme inhibition
Inhibitory Concentration 50
amylases
inhibitory concentration 50

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

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title = "Loss of Native Flavanols during Fermentation and Roasting Does Not Necessarily Reduce Digestive Enzyme-Inhibiting Bioactivities of Cocoa",
abstract = "Polyphenol profiles and in vitro digestive enzyme inhibitory activities were compared between cocoa extracts from unfermented beans (UB), fermented beans (FB), unfermented liquor (UL), and fermented liquor (FL). Total polyphenols, total flavanols, and individual flavanols were significantly different between UB/FB and UL/FL. All extracts effectively inhibited α-glucosidase (lowest IC50 = 90.0 μg/mL, UL) and moderately inhibited α-amylase (lowest IC50 = 183 μg/mL, FL) and lipase (lowest IC25 = 65.5 μg/mL, FB). Our data suggest that fermentation does not reduce α-glucosidase inhibition, while roasting may enhance inhibition. For α-amylase, both fermentation and roasting improved inhibition. Finally, for lipase, both fermentation and roasting attenuated inhibition. Conclusive correlations between inhibition and mDP, total polyphenol, and flavanol contents were not found. Our data suggest that enzyme inhibition activities of cocoa are not uniformly reduced by polyphenol/flavanol losses during fermentation and roasting. This paradigm-challenging finding suggests other cocoa constituents, potentially formed during processing, contribute to digestive enzyme inhibition.",
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Loss of Native Flavanols during Fermentation and Roasting Does Not Necessarily Reduce Digestive Enzyme-Inhibiting Bioactivities of Cocoa. / Ryan, Caroline M.; Khoo, Weslie; Ye, Liyun; Lambert, Joshua D.; Okeefe, Sean F.; Neilson, Andrew P.

In: Journal of agricultural and food chemistry, Vol. 64, No. 18, 11.05.2016, p. 3616-3625.

Research output: Contribution to journalArticle

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AU - Khoo, Weslie

AU - Ye, Liyun

AU - Lambert, Joshua D.

AU - Okeefe, Sean F.

AU - Neilson, Andrew P.

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