LOVely enzymes - Towards engineering light-controllable biocatalysts

Ulrich Krauss, Jeeyeon Lee, Stephen J. Benkovic, Karl Erich Jaeger

Research output: Contribution to journalShort survey

36 Citations (Scopus)

Abstract

Light control over enzyme function represents a novel and exciting field of biocatalysis research. Blue-light photoreceptors of the Light, Oxygen, Voltage (LOV) family have recently been investigated for their applicability as photoactive switches. We discuss here the primary photochemical events leading to light activation of LOV domains as well as the proposed signal propagation mechanism to the respective effector domain. Furthermore, we describe the construction of LOV fusions to different effector domains, namely a dihydrofolate reductase from Escherichia coli and a lipase from Bacillus subtilis. Both fusion partners retained functionality, and alteration of enzyme activity by light was also demonstrated. Hence, it appears that fusion of LOV photoreceptors to functional enzyme target sites via appropriate linker structures may represent a straightforward strategy to design light controllable biocatalysts.

Original languageEnglish (US)
Pages (from-to)15-23
Number of pages9
JournalMicrobial Biotechnology
Volume3
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

Biocatalysts
Enzymes
Light
Oxygen
Fusion reactions
Electric potential
Biocatalysis
Tetrahydrofolate Dehydrogenase
Lipases
Enzyme activity
Bacilli
Bacillus subtilis
Lipase
Escherichia coli
Chemical activation
Switches

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Krauss, Ulrich ; Lee, Jeeyeon ; Benkovic, Stephen J. ; Jaeger, Karl Erich. / LOVely enzymes - Towards engineering light-controllable biocatalysts. In: Microbial Biotechnology. 2010 ; Vol. 3, No. 1. pp. 15-23.
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LOVely enzymes - Towards engineering light-controllable biocatalysts. / Krauss, Ulrich; Lee, Jeeyeon; Benkovic, Stephen J.; Jaeger, Karl Erich.

In: Microbial Biotechnology, Vol. 3, No. 1, 01.01.2010, p. 15-23.

Research output: Contribution to journalShort survey

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AU - Krauss, Ulrich

AU - Lee, Jeeyeon

AU - Benkovic, Stephen J.

AU - Jaeger, Karl Erich

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AB - Light control over enzyme function represents a novel and exciting field of biocatalysis research. Blue-light photoreceptors of the Light, Oxygen, Voltage (LOV) family have recently been investigated for their applicability as photoactive switches. We discuss here the primary photochemical events leading to light activation of LOV domains as well as the proposed signal propagation mechanism to the respective effector domain. Furthermore, we describe the construction of LOV fusions to different effector domains, namely a dihydrofolate reductase from Escherichia coli and a lipase from Bacillus subtilis. Both fusion partners retained functionality, and alteration of enzyme activity by light was also demonstrated. Hence, it appears that fusion of LOV photoreceptors to functional enzyme target sites via appropriate linker structures may represent a straightforward strategy to design light controllable biocatalysts.

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