Lower prevalence of antibiotic-resistant Salmonella on large-scale U.S. conventional poultry farms that transitioned to organic practices

Amy R. Sapkota, Erinna L. Kinney, Ashish George, R. Michael Hulet, Raul Cruz-Cano, Kellogg J. Schwab, Guangyu Zhang, Sam W. Joseph

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

As a result of the widespread use of antibiotics in large-scale U.S. poultry production, a significant proportion of Salmonella strains recovered from conventional poultry farms and retail poultry products express antibiotic resistance. We evaluated whether large-scale poultry farms that transitioned from conventional to organic practices and discontinued antibiotic use were characterized by differences in the prevalence of antibiotic-resistant Salmonella compared to farms that maintained conventional practices. We collected poultry litter, water and feed samples from 10 newly organic and 10 conventional poultry houses. Samples were analyzed for Salmonella using standard enrichment methods. Isolates were confirmed using standard biochemical tests and the Vitek®2 Compact System. Antimicrobial susceptibility testing was performed by Sensititre® microbroth dilution. Data were analyzed using Fisher's exact test and generalized linear mixed models. We detected Salmonella in both conventional and newly organic poultry houses. Salmonella Kentucky was the predominant serovar identified, followed by S. Orion, S. Enteritidis, S. Gostrup and S. Infantis. Among S. Kentucky isolates (n = 41), percent resistance was statistically significantly lower among isolates recovered from newly organic versus conventional poultry houses for: amoxicillin-clavulanate ( p= 0.049), ampicillin ( p= 0.042), cefoxitin ( p= 0.042), ceftiofur ( p= 0.043) and ceftriaxone ( p= 0.042). Percent multidrug resistance (resistance to ≥. 3 antimicrobial classes) was also statistically significantly lower among S. Kentucky isolates recovered from newly organic poultry houses (6%) compared to those recovered from conventional houses (44%) ( p= 0.015). To our knowledge, these are the first U.S. data to show immediate, on-farm changes in the prevalence of antibiotic-resistant Salmonella when antibiotics are voluntarily withdrawn from large-scale poultry facilities in the United States.

Original languageEnglish (US)
Pages (from-to)387-392
Number of pages6
JournalScience of the Total Environment
Volume476-477
DOIs
StatePublished - Apr 1 2014

Fingerprint

Poultry
Salmonella
Antibiotics
poultry
antibiotics
Farms
farm
Anti-Bacterial Agents
salmonella
Cefoxitin
Clavulanic Acid
Ceftriaxone
antibiotic resistance
Amoxicillin
Ampicillin
Dilution
litter
dilution

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

Sapkota, Amy R. ; Kinney, Erinna L. ; George, Ashish ; Hulet, R. Michael ; Cruz-Cano, Raul ; Schwab, Kellogg J. ; Zhang, Guangyu ; Joseph, Sam W. / Lower prevalence of antibiotic-resistant Salmonella on large-scale U.S. conventional poultry farms that transitioned to organic practices. In: Science of the Total Environment. 2014 ; Vol. 476-477. pp. 387-392.
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Lower prevalence of antibiotic-resistant Salmonella on large-scale U.S. conventional poultry farms that transitioned to organic practices. / Sapkota, Amy R.; Kinney, Erinna L.; George, Ashish; Hulet, R. Michael; Cruz-Cano, Raul; Schwab, Kellogg J.; Zhang, Guangyu; Joseph, Sam W.

In: Science of the Total Environment, Vol. 476-477, 01.04.2014, p. 387-392.

Research output: Contribution to journalArticle

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AB - As a result of the widespread use of antibiotics in large-scale U.S. poultry production, a significant proportion of Salmonella strains recovered from conventional poultry farms and retail poultry products express antibiotic resistance. We evaluated whether large-scale poultry farms that transitioned from conventional to organic practices and discontinued antibiotic use were characterized by differences in the prevalence of antibiotic-resistant Salmonella compared to farms that maintained conventional practices. We collected poultry litter, water and feed samples from 10 newly organic and 10 conventional poultry houses. Samples were analyzed for Salmonella using standard enrichment methods. Isolates were confirmed using standard biochemical tests and the Vitek®2 Compact System. Antimicrobial susceptibility testing was performed by Sensititre® microbroth dilution. Data were analyzed using Fisher's exact test and generalized linear mixed models. We detected Salmonella in both conventional and newly organic poultry houses. Salmonella Kentucky was the predominant serovar identified, followed by S. Orion, S. Enteritidis, S. Gostrup and S. Infantis. Among S. Kentucky isolates (n = 41), percent resistance was statistically significantly lower among isolates recovered from newly organic versus conventional poultry houses for: amoxicillin-clavulanate ( p= 0.049), ampicillin ( p= 0.042), cefoxitin ( p= 0.042), ceftiofur ( p= 0.043) and ceftriaxone ( p= 0.042). Percent multidrug resistance (resistance to ≥. 3 antimicrobial classes) was also statistically significantly lower among S. Kentucky isolates recovered from newly organic poultry houses (6%) compared to those recovered from conventional houses (44%) ( p= 0.015). To our knowledge, these are the first U.S. data to show immediate, on-farm changes in the prevalence of antibiotic-resistant Salmonella when antibiotics are voluntarily withdrawn from large-scale poultry facilities in the United States.

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