Luminal regulation of Na +/H + exchanger gene expression in rat ileal mucosa

Marc A. Doble, Vicky B. Tola, Stephanie A. Chamberlain, Robert R. Cima, Alfred Van Hoek, David I. Soybel

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

It is well recognized that ileostomy patients suffer from chronic depletion of Na + through the stoma effluent. In this study we evaluated the effects of ileostomy on messenger RNA levels that encode different Na +/H + exchanger isoforms (NHE-2 and NHE-3). Loop ileostomies were created in Sprague-Dawley rats. Segments of diverted ileum were harvested for quantitation of mRNA levels encoding these isoforms and the Na +/K + ATPase in mucosal scrapings and for immunofluorescence microscopy, specifically of the NHE-3 protein. Our studies indicate that as early as 8 days after diversion, NHE-3 gene expression is selectively attenuated in poststomal ileal mucosa. Mucosal morphology remains undisturbed, and the distribution of protein expression along the crypt/villus axis is not altered. Infusion of Na + or the enterocyte nutrient, glutamine, into the lumen of the diverted segment restores or even augments mRNA levels for NHE-3, again without altering the histologic appearance or distribution of the protein along the crypt/villus axis. These effects are specific because nonpolar osmolytes (mannitol) and related organic nutrients not specific for the enterocyte (i.e., butyrate) have no effect on mRNA levels of NHE-3. Further work is required to understand how the early changes in mRNA contribute to mucosal function and response to luminal diversion.

Original languageEnglish (US)
Pages (from-to)387-395
Number of pages9
JournalJournal of Gastrointestinal Surgery
Volume6
Issue number3
DOIs
StatePublished - Dec 1 2002

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Sodium-Hydrogen Antiporter
Mucous Membrane
Ileostomy
Gene Expression
Messenger RNA
Enterocytes
Protein Isoforms
Food
Butyrates
Mannitol
Glutamine
Ileum
Fluorescence Microscopy
Sprague Dawley Rats
Proteins

All Science Journal Classification (ASJC) codes

  • Surgery
  • Gastroenterology

Cite this

Doble, M. A., Tola, V. B., Chamberlain, S. A., Cima, R. R., Van Hoek, A., & Soybel, D. I. (2002). Luminal regulation of Na +/H + exchanger gene expression in rat ileal mucosa. Journal of Gastrointestinal Surgery, 6(3), 387-395. https://doi.org/10.1016/S1091-255X(01)00049-X
Doble, Marc A. ; Tola, Vicky B. ; Chamberlain, Stephanie A. ; Cima, Robert R. ; Van Hoek, Alfred ; Soybel, David I. / Luminal regulation of Na +/H + exchanger gene expression in rat ileal mucosa. In: Journal of Gastrointestinal Surgery. 2002 ; Vol. 6, No. 3. pp. 387-395.
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Doble, MA, Tola, VB, Chamberlain, SA, Cima, RR, Van Hoek, A & Soybel, DI 2002, 'Luminal regulation of Na +/H + exchanger gene expression in rat ileal mucosa', Journal of Gastrointestinal Surgery, vol. 6, no. 3, pp. 387-395. https://doi.org/10.1016/S1091-255X(01)00049-X

Luminal regulation of Na +/H + exchanger gene expression in rat ileal mucosa. / Doble, Marc A.; Tola, Vicky B.; Chamberlain, Stephanie A.; Cima, Robert R.; Van Hoek, Alfred; Soybel, David I.

In: Journal of Gastrointestinal Surgery, Vol. 6, No. 3, 01.12.2002, p. 387-395.

Research output: Contribution to journalArticle

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AU - Soybel, David I.

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