Lung alveolar wall disruption in three-dimensional space identified using second-harmonic generation and multiphoton excitation fluorescence

Thomas Abraham, James Hogg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Second harmonic generation and multiphoton excited fluorescence microscopy methods were used to examine structural remodeling of the extracellular matrix in human lung alveolar walls undergoing emphysematous destruction. Fresh lung samples removed from a patient undergoing lung transplantation for very severe chronic obstructive pulmonary disease were compared to similar samples from an unused donor lung that served as a control. The generated spatially resolved 3D images show the spatial distribution of collagen, elastin and other endogenously fluorescent tissue components such as macrophages. In the case of control lung tissue, we found well ordered alveolar walls with composite type structure made up of collagen matrix and relatively fine elastic fibers. In contrast, lung tissue undergoing emphysematous destruction was highly disorganized with increased alveolar wall thickness compared to control lung tissue.

Original languageEnglish (US)
Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VIII
DOIs
StatePublished - 2010
EventAdvanced Biomedical and Clinical Diagnostic Systems VIII - San Francisco, CA, United States
Duration: Jan 24 2010Jan 26 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7555
ISSN (Print)1605-7422

Other

OtherAdvanced Biomedical and Clinical Diagnostic Systems VIII
CountryUnited States
CitySan Francisco, CA
Period1/24/101/26/10

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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