Luteal cells from functional and regressing bovine corpora lutea differentially alter the function of gamma delta T cells

Sadhat S. Walusimbi, Joy Lee Pate

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The luteal microenvironment is thought to direct the function of resident immune cells to facilitate either luteal function or regression. To determine if luteal cells from functional (Days 10- 12) and regressing (8 h after administration of prostaglandin F2alpha) corpora lutea (CL) induce different responses in γδ T cells, luteal cells were cocultured with autologous γδ T cells isolated from peripheral blood. Proliferation, functional phenotypes, and cytokine synthesis were analyzed by flow cytometry. To determine if the luteal cells from functional CL induce hyporesponsiveness in γδ T cells, γδ+ cells were cocultured with midcycle luteal cells and further stimulated with concanavalin A. Coculture of γδ+ cells with midcycle luteal cells did not inhibit concanavalin A-induced proliferation. In a proliferation assay, luteal cells from midcycle CL predominantly induced proliferation of γδ+ WC1- cells (P<0.05), while luteal cells from regressing CL predominantly induced proliferation of γδ+WC1+ cells (P<0.05). Analysis of intracellular cytokines indicated that midcycle luteal cells increased the proportion of γδ+ cells containing interleukin 10 (P<0.05), but reduced the proportion of γδ+ cells containing interferon gamma (IFNG; P<0.05). There were no changes in the proportions of γδ+ cells synthesizing interleukin 4 or tumor necrosis factor. Unexpectedly, coculture of γδ+ cells with luteal cells from regressing CL had no effect on any of the cytokines analyzed. These data support the hypothesis that the function of resident T cells is differentially modulated depending on the status of the CL.

Original languageEnglish (US)
Article number140
JournalBiology of Reproduction
Volume90
Issue number6
DOIs
StatePublished - Jan 1 2014

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Luteal Cells
Somatostatin-Secreting Cells
Corpus Luteum
T-Lymphocytes
Cytokines
Concanavalin A
Coculture Techniques
Cell Proliferation
Dinoprost
Interleukin-4
Interleukin-10
Interferon-gamma
Flow Cytometry
Tumor Necrosis Factor-alpha
Phenotype

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Medicine(all)

Cite this

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abstract = "The luteal microenvironment is thought to direct the function of resident immune cells to facilitate either luteal function or regression. To determine if luteal cells from functional (Days 10- 12) and regressing (8 h after administration of prostaglandin F2alpha) corpora lutea (CL) induce different responses in γδ T cells, luteal cells were cocultured with autologous γδ T cells isolated from peripheral blood. Proliferation, functional phenotypes, and cytokine synthesis were analyzed by flow cytometry. To determine if the luteal cells from functional CL induce hyporesponsiveness in γδ T cells, γδ+ cells were cocultured with midcycle luteal cells and further stimulated with concanavalin A. Coculture of γδ+ cells with midcycle luteal cells did not inhibit concanavalin A-induced proliferation. In a proliferation assay, luteal cells from midcycle CL predominantly induced proliferation of γδ+ WC1- cells (P<0.05), while luteal cells from regressing CL predominantly induced proliferation of γδ+WC1+ cells (P<0.05). Analysis of intracellular cytokines indicated that midcycle luteal cells increased the proportion of γδ+ cells containing interleukin 10 (P<0.05), but reduced the proportion of γδ+ cells containing interferon gamma (IFNG; P<0.05). There were no changes in the proportions of γδ+ cells synthesizing interleukin 4 or tumor necrosis factor. Unexpectedly, coculture of γδ+ cells with luteal cells from regressing CL had no effect on any of the cytokines analyzed. These data support the hypothesis that the function of resident T cells is differentially modulated depending on the status of the CL.",
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Luteal cells from functional and regressing bovine corpora lutea differentially alter the function of gamma delta T cells. / Walusimbi, Sadhat S.; Pate, Joy Lee.

In: Biology of Reproduction, Vol. 90, No. 6, 140, 01.01.2014.

Research output: Contribution to journalArticle

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