Luteal microenvironment directs resident T lymphocyte function in cows

Daniel H. Poole, Joy Lee Pate

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The corpus luteum (CL) is a transient endocrine organ composed of a heterogeneous mixture of cells. Functional interactions exist between peripheral T cells and luteal cells in vitro; however, the precise role of resident T cells (RTC) remains unknown. The goals of the present study were to isolate RTC from within the CL and determine if alteration of luteal function resulted in changes in RTC phenotypes. Functional lymphocyte phenotypes identified in the bovine CL by using quantitative flow cytometric analysis were clearly different from those in the peripheral circulation. The proportion of CD8+ RTC was greater than CD4+ RTC. These proportions were opposite in peripheral blood. The proportion of cd+ lymphocytes was not different in the CL compared to that in peripheral blood nor was it altered during luteal regression. There was a significant increase in CD8αα+ and γδ+CD8αα+ RTC during luteal regression. The proportion of FOXP3+ lymphocytes in the CL was greater than that isolated from peripheral blood, and this proportion of lymphocytes was dramatically reduced by induction of luteolysis. Within the CL of early pregnancy, there was an increase in the CD8αβ+ and γδ+CD8αβ+ populations compared to those in the CL of nonpregnant animals. Based on these data, we concluded that the functional state of the CL creates a microenvironment that regulates the recruitment of or differentiation into specific lymphocyte types. Understanding the interactions between steroidogenic cells and ovarian lymphocytes within CL will not only enhance understanding of reproductive function but may provide vital clues to lymphocyte regulation within tissues.

Original languageEnglish (US)
Article number29
JournalBiology of reproduction
Volume86
Issue number2
DOIs
StatePublished - Feb 1 2012

Fingerprint

Corpus Luteum
T-Lymphocytes
Lymphocytes
Luteolysis
Phenotype
Luteal Cells
Pregnancy

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Cell Biology

Cite this

@article{5e310f8761d44006a5cf0de5f65c8fbe,
title = "Luteal microenvironment directs resident T lymphocyte function in cows",
abstract = "The corpus luteum (CL) is a transient endocrine organ composed of a heterogeneous mixture of cells. Functional interactions exist between peripheral T cells and luteal cells in vitro; however, the precise role of resident T cells (RTC) remains unknown. The goals of the present study were to isolate RTC from within the CL and determine if alteration of luteal function resulted in changes in RTC phenotypes. Functional lymphocyte phenotypes identified in the bovine CL by using quantitative flow cytometric analysis were clearly different from those in the peripheral circulation. The proportion of CD8+ RTC was greater than CD4+ RTC. These proportions were opposite in peripheral blood. The proportion of cd+ lymphocytes was not different in the CL compared to that in peripheral blood nor was it altered during luteal regression. There was a significant increase in CD8αα+ and γδ+CD8αα+ RTC during luteal regression. The proportion of FOXP3+ lymphocytes in the CL was greater than that isolated from peripheral blood, and this proportion of lymphocytes was dramatically reduced by induction of luteolysis. Within the CL of early pregnancy, there was an increase in the CD8αβ+ and γδ+CD8αβ+ populations compared to those in the CL of nonpregnant animals. Based on these data, we concluded that the functional state of the CL creates a microenvironment that regulates the recruitment of or differentiation into specific lymphocyte types. Understanding the interactions between steroidogenic cells and ovarian lymphocytes within CL will not only enhance understanding of reproductive function but may provide vital clues to lymphocyte regulation within tissues.",
author = "Poole, {Daniel H.} and Pate, {Joy Lee}",
year = "2012",
month = "2",
day = "1",
doi = "10.1095/biolreprod.111.092296",
language = "English (US)",
volume = "86",
journal = "Biology of Reproduction",
issn = "0006-3363",
publisher = "Society for the Study of Reproduction",
number = "2",

}

Luteal microenvironment directs resident T lymphocyte function in cows. / Poole, Daniel H.; Pate, Joy Lee.

In: Biology of reproduction, Vol. 86, No. 2, 29, 01.02.2012.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Luteal microenvironment directs resident T lymphocyte function in cows

AU - Poole, Daniel H.

AU - Pate, Joy Lee

PY - 2012/2/1

Y1 - 2012/2/1

N2 - The corpus luteum (CL) is a transient endocrine organ composed of a heterogeneous mixture of cells. Functional interactions exist between peripheral T cells and luteal cells in vitro; however, the precise role of resident T cells (RTC) remains unknown. The goals of the present study were to isolate RTC from within the CL and determine if alteration of luteal function resulted in changes in RTC phenotypes. Functional lymphocyte phenotypes identified in the bovine CL by using quantitative flow cytometric analysis were clearly different from those in the peripheral circulation. The proportion of CD8+ RTC was greater than CD4+ RTC. These proportions were opposite in peripheral blood. The proportion of cd+ lymphocytes was not different in the CL compared to that in peripheral blood nor was it altered during luteal regression. There was a significant increase in CD8αα+ and γδ+CD8αα+ RTC during luteal regression. The proportion of FOXP3+ lymphocytes in the CL was greater than that isolated from peripheral blood, and this proportion of lymphocytes was dramatically reduced by induction of luteolysis. Within the CL of early pregnancy, there was an increase in the CD8αβ+ and γδ+CD8αβ+ populations compared to those in the CL of nonpregnant animals. Based on these data, we concluded that the functional state of the CL creates a microenvironment that regulates the recruitment of or differentiation into specific lymphocyte types. Understanding the interactions between steroidogenic cells and ovarian lymphocytes within CL will not only enhance understanding of reproductive function but may provide vital clues to lymphocyte regulation within tissues.

AB - The corpus luteum (CL) is a transient endocrine organ composed of a heterogeneous mixture of cells. Functional interactions exist between peripheral T cells and luteal cells in vitro; however, the precise role of resident T cells (RTC) remains unknown. The goals of the present study were to isolate RTC from within the CL and determine if alteration of luteal function resulted in changes in RTC phenotypes. Functional lymphocyte phenotypes identified in the bovine CL by using quantitative flow cytometric analysis were clearly different from those in the peripheral circulation. The proportion of CD8+ RTC was greater than CD4+ RTC. These proportions were opposite in peripheral blood. The proportion of cd+ lymphocytes was not different in the CL compared to that in peripheral blood nor was it altered during luteal regression. There was a significant increase in CD8αα+ and γδ+CD8αα+ RTC during luteal regression. The proportion of FOXP3+ lymphocytes in the CL was greater than that isolated from peripheral blood, and this proportion of lymphocytes was dramatically reduced by induction of luteolysis. Within the CL of early pregnancy, there was an increase in the CD8αβ+ and γδ+CD8αβ+ populations compared to those in the CL of nonpregnant animals. Based on these data, we concluded that the functional state of the CL creates a microenvironment that regulates the recruitment of or differentiation into specific lymphocyte types. Understanding the interactions between steroidogenic cells and ovarian lymphocytes within CL will not only enhance understanding of reproductive function but may provide vital clues to lymphocyte regulation within tissues.

UR - http://www.scopus.com/inward/record.url?scp=84860238692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860238692&partnerID=8YFLogxK

U2 - 10.1095/biolreprod.111.092296

DO - 10.1095/biolreprod.111.092296

M3 - Article

C2 - 21976598

AN - SCOPUS:84860238692

VL - 86

JO - Biology of Reproduction

JF - Biology of Reproduction

SN - 0006-3363

IS - 2

M1 - 29

ER -