LuxS coexpression enhances yields of recombinant proteins in Escherichia coli in part through posttranscriptional control of GroEL

Chen Yu Tsao, Liang Wang, Yoshifumi Hashimoto, Hyunmin Yi, John C. March, Matthew P. DeLisa, Thomas K. Wood, James J. Valdes, William E. Bentley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cell-to-cell communication, or quorum sensing (QS), enables cell density-dependent regulation of bacterial gene expression which can be exploited for the autonomous-signal-guided expression of recombinant proteins (C. Y. Tsao, S. Hooshangi, H. C. Wu, J. J. Valdes, and W. E. Bentley, Metab. Eng. 12:291-297, 2010). Earlier observations that the metabolic potential of Escherichia coli is conveyed via the QS signaling molecule autoinducer-2 (AI-2) suggested that the capacity for protein synthesis could also be affected by AI-2 signaling (M. P. DeLisa, J. J. Valdes, and W. E. Bentley, J. Bacteriol. 183:2918-2928, 2001). In this work, we found that simply adding conditioned medium containing high levels of AI-2 at the same time as inducing the synthesis of recombinant proteins doubled the yield of active product. We have hypothesized that AI-2 signaling "conditions" cells as a natural consequence of cell-to-cell communication and that this could tweak the signal transduction cascade to alter the protein synthesis landscape. We inserted luxS (AI-2 synthase) into vectors which cosynthesized proteins of interest (organophosphorus hydrolase [OPH], chloramphenicol acetyltransferase [CAT], or UV-variant green fluorescent protein [GFPuv]) and evaluated the protein expression in luxS-deficient hosts. In this way, we altered the level of luxS in the cells in order to "tune" the synthesis of AI-2. We found conditions in which the protein yield was dramatically increased. Further studies demonstrated coincident upregulation of the chaperone GroEL, which may have facilitated higher yields and is shown for the first time to be positively regulated at the posttranscriptional level by AI-2. This report is the first to demonstrate that the protein synthesis capacity of E. coli can be altered by rewiring quorum sensing circuitry.

Original languageEnglish (US)
Pages (from-to)2141-2152
Number of pages12
JournalApplied and environmental microbiology
Volume77
Issue number6
DOIs
StatePublished - Mar 1 2011

Fingerprint

Recombinant Proteins
recombinant proteins
quorum sensing
protein synthesis
Escherichia coli
cell communication
protein
Quorum Sensing
Proteins
chloramphenicol acetyltransferase
synthesis
Cell Communication
Bacterial Gene Expression Regulation
cells
green fluorescent protein
signal transduction
proteins
Aryldialkylphosphatase
Chloramphenicol O-Acetyltransferase
communication

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Tsao, Chen Yu ; Wang, Liang ; Hashimoto, Yoshifumi ; Yi, Hyunmin ; March, John C. ; DeLisa, Matthew P. ; Wood, Thomas K. ; Valdes, James J. ; Bentley, William E. / LuxS coexpression enhances yields of recombinant proteins in Escherichia coli in part through posttranscriptional control of GroEL. In: Applied and environmental microbiology. 2011 ; Vol. 77, No. 6. pp. 2141-2152.
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LuxS coexpression enhances yields of recombinant proteins in Escherichia coli in part through posttranscriptional control of GroEL. / Tsao, Chen Yu; Wang, Liang; Hashimoto, Yoshifumi; Yi, Hyunmin; March, John C.; DeLisa, Matthew P.; Wood, Thomas K.; Valdes, James J.; Bentley, William E.

In: Applied and environmental microbiology, Vol. 77, No. 6, 01.03.2011, p. 2141-2152.

Research output: Contribution to journalArticle

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AU - Tsao, Chen Yu

AU - Wang, Liang

AU - Hashimoto, Yoshifumi

AU - Yi, Hyunmin

AU - March, John C.

AU - DeLisa, Matthew P.

AU - Wood, Thomas K.

AU - Valdes, James J.

AU - Bentley, William E.

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