Lymphocyte proliferation response of lactating dairy cows fed varying concentrations of rumen-protected methionine

K. J. Soder, Lisa Holden

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Six midlactation Holstein cows were used in a replicated 3 × 3 Latin square design to characterize the influence of dietary concentration of Mepron®85 (Degussa Corp., Allendale, NJ) on isolated mononuclear cell composition and lymphocyte proliferation. Cows were fed a common total mixed ration containing corn silage, legume silage, chopped legume hay, and a grain and mineral pellet that was top-dressed with one of three treatments. Treatments consisted of 1) 0 g/d of Mepron®85, 2) 15 g/d of Mepron®85 (11 g of rumen-protected Met), or 3) 30 g/d of Mepron®85 (22 g of rumen-protected Met). Cows were housed in a tie-stall barn, had continuous access to fresh water, and were fed once daily at 0900 h for ad libitum intake. Dry matter intake (DMI), orts, and milk yields were recorded daily, and weekly milk samples were collected for analyses of fat, protein, SCC, and milk urea nitrogen. Blood and milk samples were collected before the beginning of the experiment and during wk 2 and 4 of each 28-d treatment period. Blood was analyzed for serum methionine, lymphocyte proliferation, and phenotypic composition of isolated mononuclear cells. Milk samples were analyzed for phenotypic composition of isolated mononuclear cells. Least square means for DMI, milk yield, milk composition, and phenotypic mononuclear cell composition of blood and milk were not affected by treatment. Proliferative ability of peripheral blood T lymphocytes increased for cows consuming 30 g/d of Mepron®85.

Original languageEnglish (US)
Pages (from-to)1935-1942
Number of pages8
JournalJournal of dairy science
Volume82
Issue number9
DOIs
StatePublished - Jan 1 1999

Fingerprint

Rumen
lymphocyte proliferation
Methionine
methionine
rumen
Milk
dairy cows
Lymphocytes
cows
milk
milk composition
dry matter intake
milk yield
blood
legumes
cells
blood composition
Silage
tie stalls
total mixed rations

All Science Journal Classification (ASJC) codes

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

@article{03165f9e1af3491d84cdbebc243294d9,
title = "Lymphocyte proliferation response of lactating dairy cows fed varying concentrations of rumen-protected methionine",
abstract = "Six midlactation Holstein cows were used in a replicated 3 × 3 Latin square design to characterize the influence of dietary concentration of Mepron{\circledR}85 (Degussa Corp., Allendale, NJ) on isolated mononuclear cell composition and lymphocyte proliferation. Cows were fed a common total mixed ration containing corn silage, legume silage, chopped legume hay, and a grain and mineral pellet that was top-dressed with one of three treatments. Treatments consisted of 1) 0 g/d of Mepron{\circledR}85, 2) 15 g/d of Mepron{\circledR}85 (11 g of rumen-protected Met), or 3) 30 g/d of Mepron{\circledR}85 (22 g of rumen-protected Met). Cows were housed in a tie-stall barn, had continuous access to fresh water, and were fed once daily at 0900 h for ad libitum intake. Dry matter intake (DMI), orts, and milk yields were recorded daily, and weekly milk samples were collected for analyses of fat, protein, SCC, and milk urea nitrogen. Blood and milk samples were collected before the beginning of the experiment and during wk 2 and 4 of each 28-d treatment period. Blood was analyzed for serum methionine, lymphocyte proliferation, and phenotypic composition of isolated mononuclear cells. Milk samples were analyzed for phenotypic composition of isolated mononuclear cells. Least square means for DMI, milk yield, milk composition, and phenotypic mononuclear cell composition of blood and milk were not affected by treatment. Proliferative ability of peripheral blood T lymphocytes increased for cows consuming 30 g/d of Mepron{\circledR}85.",
author = "Soder, {K. J.} and Lisa Holden",
year = "1999",
month = "1",
day = "1",
doi = "10.3168/jds.S0022-0302(99)75429-9",
language = "English (US)",
volume = "82",
pages = "1935--1942",
journal = "Journal of Dairy Science",
issn = "0022-0302",
publisher = "Elsevier Limited",
number = "9",

}

Lymphocyte proliferation response of lactating dairy cows fed varying concentrations of rumen-protected methionine. / Soder, K. J.; Holden, Lisa.

In: Journal of dairy science, Vol. 82, No. 9, 01.01.1999, p. 1935-1942.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Lymphocyte proliferation response of lactating dairy cows fed varying concentrations of rumen-protected methionine

AU - Soder, K. J.

AU - Holden, Lisa

PY - 1999/1/1

Y1 - 1999/1/1

N2 - Six midlactation Holstein cows were used in a replicated 3 × 3 Latin square design to characterize the influence of dietary concentration of Mepron®85 (Degussa Corp., Allendale, NJ) on isolated mononuclear cell composition and lymphocyte proliferation. Cows were fed a common total mixed ration containing corn silage, legume silage, chopped legume hay, and a grain and mineral pellet that was top-dressed with one of three treatments. Treatments consisted of 1) 0 g/d of Mepron®85, 2) 15 g/d of Mepron®85 (11 g of rumen-protected Met), or 3) 30 g/d of Mepron®85 (22 g of rumen-protected Met). Cows were housed in a tie-stall barn, had continuous access to fresh water, and were fed once daily at 0900 h for ad libitum intake. Dry matter intake (DMI), orts, and milk yields were recorded daily, and weekly milk samples were collected for analyses of fat, protein, SCC, and milk urea nitrogen. Blood and milk samples were collected before the beginning of the experiment and during wk 2 and 4 of each 28-d treatment period. Blood was analyzed for serum methionine, lymphocyte proliferation, and phenotypic composition of isolated mononuclear cells. Milk samples were analyzed for phenotypic composition of isolated mononuclear cells. Least square means for DMI, milk yield, milk composition, and phenotypic mononuclear cell composition of blood and milk were not affected by treatment. Proliferative ability of peripheral blood T lymphocytes increased for cows consuming 30 g/d of Mepron®85.

AB - Six midlactation Holstein cows were used in a replicated 3 × 3 Latin square design to characterize the influence of dietary concentration of Mepron®85 (Degussa Corp., Allendale, NJ) on isolated mononuclear cell composition and lymphocyte proliferation. Cows were fed a common total mixed ration containing corn silage, legume silage, chopped legume hay, and a grain and mineral pellet that was top-dressed with one of three treatments. Treatments consisted of 1) 0 g/d of Mepron®85, 2) 15 g/d of Mepron®85 (11 g of rumen-protected Met), or 3) 30 g/d of Mepron®85 (22 g of rumen-protected Met). Cows were housed in a tie-stall barn, had continuous access to fresh water, and were fed once daily at 0900 h for ad libitum intake. Dry matter intake (DMI), orts, and milk yields were recorded daily, and weekly milk samples were collected for analyses of fat, protein, SCC, and milk urea nitrogen. Blood and milk samples were collected before the beginning of the experiment and during wk 2 and 4 of each 28-d treatment period. Blood was analyzed for serum methionine, lymphocyte proliferation, and phenotypic composition of isolated mononuclear cells. Milk samples were analyzed for phenotypic composition of isolated mononuclear cells. Least square means for DMI, milk yield, milk composition, and phenotypic mononuclear cell composition of blood and milk were not affected by treatment. Proliferative ability of peripheral blood T lymphocytes increased for cows consuming 30 g/d of Mepron®85.

UR - http://www.scopus.com/inward/record.url?scp=0033192825&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033192825&partnerID=8YFLogxK

U2 - 10.3168/jds.S0022-0302(99)75429-9

DO - 10.3168/jds.S0022-0302(99)75429-9

M3 - Article

VL - 82

SP - 1935

EP - 1942

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

IS - 9

ER -