Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection

A nested cross-sectional study

Mingdong Zhang, Thomas R. O'Brien, William C. Kopp, James J. Goedert, P. S. Rosenberg, C. S. Rabkin, E. A. Engels, D. Whitby, M. Elaine Eyster, B. Konkle, M. Manco-Johnson, D. DiMichele, M. W. Hilgartner, P. Blatt, L. M. Aledort, S. Seremetes, K. Hoots, A. L. Angiolillo, N. L.C. Luban, A. Cohen & 17 others C. S. Manno, C. Leissinger, G. C. White, M. M. Lederman, S. Purvis, J. Salkowitz, C. M. Kessler, A. Karafoulidou, T. Mandalaki, A. Hatzakis, G. Touloumi, W. Schramm, F. Rommel, P. de Moerloose, S. Eichinger, K. E. Sherman, B. L. Kroner

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    Background: With chronic infection, hepatitis C virus (HCV) RNA can be detected in B cells and associated with B-cell disorders, but these are not well defined. Methods: The relationship between HCV infection and lymphocyte subpopulations was evaluated rigorously in 120 asymptomatic hemophilic patients, randomly selected from a prospective cohort study. CD4+ T cells, CD8+ T cells, CD19+ B cells, and CD56+ NK cells were quantified by flow cytometry using cryopreserved peripheral blood mononuclear cells of 24 hemophilic patients in each of five age-matched groups [uninfected; chronic HCV with or without human immunodeficiency virus (HIV); and cleared HCV with or without HIV]. Results: As expected, patients with HIV had significantly reduced CD4+ and increased CD8+ T cells. Irrespective of HIV, patients with chronic HCV infection had approximately 25% fewer CD19+ B cells than those without chronic HCV infection. Conclusions: These data support the hypothesis that asymptomatic patients with chronic HCV infection have an altered B-lymphocyte population.

    Original languageEnglish (US)
    JournalBMC Blood Disorders
    Volume5
    DOIs
    StatePublished - Jan 18 2005

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    Lymphocyte Subsets
    Virus Diseases
    Coinfection
    Hepacivirus
    Cross-Sectional Studies
    Chronic Hepatitis C
    HIV
    B-Lymphocytes
    T-Lymphocytes
    Natural Killer Cells
    Blood Cells
    Flow Cytometry
    Cohort Studies
    Research Design
    Age Groups
    Prospective Studies
    RNA
    Infection
    Population

    All Science Journal Classification (ASJC) codes

    • Hematology
    • Molecular Biology

    Cite this

    Zhang, Mingdong ; O'Brien, Thomas R. ; Kopp, William C. ; Goedert, James J. ; Rosenberg, P. S. ; Rabkin, C. S. ; Engels, E. A. ; Whitby, D. ; Eyster, M. Elaine ; Konkle, B. ; Manco-Johnson, M. ; DiMichele, D. ; Hilgartner, M. W. ; Blatt, P. ; Aledort, L. M. ; Seremetes, S. ; Hoots, K. ; Angiolillo, A. L. ; Luban, N. L.C. ; Cohen, A. ; Manno, C. S. ; Leissinger, C. ; White, G. C. ; Lederman, M. M. ; Purvis, S. ; Salkowitz, J. ; Kessler, C. M. ; Karafoulidou, A. ; Mandalaki, T. ; Hatzakis, A. ; Touloumi, G. ; Schramm, W. ; Rommel, F. ; de Moerloose, P. ; Eichinger, S. ; Sherman, K. E. ; Kroner, B. L. / Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection : A nested cross-sectional study. In: BMC Blood Disorders. 2005 ; Vol. 5.
    @article{0c6609ac29c045b2b90c321715d9a9c1,
    title = "Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection: A nested cross-sectional study",
    abstract = "Background: With chronic infection, hepatitis C virus (HCV) RNA can be detected in B cells and associated with B-cell disorders, but these are not well defined. Methods: The relationship between HCV infection and lymphocyte subpopulations was evaluated rigorously in 120 asymptomatic hemophilic patients, randomly selected from a prospective cohort study. CD4+ T cells, CD8+ T cells, CD19+ B cells, and CD56+ NK cells were quantified by flow cytometry using cryopreserved peripheral blood mononuclear cells of 24 hemophilic patients in each of five age-matched groups [uninfected; chronic HCV with or without human immunodeficiency virus (HIV); and cleared HCV with or without HIV]. Results: As expected, patients with HIV had significantly reduced CD4+ and increased CD8+ T cells. Irrespective of HIV, patients with chronic HCV infection had approximately 25{\%} fewer CD19+ B cells than those without chronic HCV infection. Conclusions: These data support the hypothesis that asymptomatic patients with chronic HCV infection have an altered B-lymphocyte population.",
    author = "Mingdong Zhang and O'Brien, {Thomas R.} and Kopp, {William C.} and Goedert, {James J.} and Rosenberg, {P. S.} and Rabkin, {C. S.} and Engels, {E. A.} and D. Whitby and Eyster, {M. Elaine} and B. Konkle and M. Manco-Johnson and D. DiMichele and Hilgartner, {M. W.} and P. Blatt and Aledort, {L. M.} and S. Seremetes and K. Hoots and Angiolillo, {A. L.} and Luban, {N. L.C.} and A. Cohen and Manno, {C. S.} and C. Leissinger and White, {G. C.} and Lederman, {M. M.} and S. Purvis and J. Salkowitz and Kessler, {C. M.} and A. Karafoulidou and T. Mandalaki and A. Hatzakis and G. Touloumi and W. Schramm and F. Rommel and {de Moerloose}, P. and S. Eichinger and Sherman, {K. E.} and Kroner, {B. L.}",
    year = "2005",
    month = "1",
    day = "18",
    doi = "10.1186/1471-2326-5-2",
    language = "English (US)",
    volume = "5",
    journal = "BMC Hematology",
    issn = "1471-2326",
    publisher = "BioMed Central",

    }

    Zhang, M, O'Brien, TR, Kopp, WC, Goedert, JJ, Rosenberg, PS, Rabkin, CS, Engels, EA, Whitby, D, Eyster, ME, Konkle, B, Manco-Johnson, M, DiMichele, D, Hilgartner, MW, Blatt, P, Aledort, LM, Seremetes, S, Hoots, K, Angiolillo, AL, Luban, NLC, Cohen, A, Manno, CS, Leissinger, C, White, GC, Lederman, MM, Purvis, S, Salkowitz, J, Kessler, CM, Karafoulidou, A, Mandalaki, T, Hatzakis, A, Touloumi, G, Schramm, W, Rommel, F, de Moerloose, P, Eichinger, S, Sherman, KE & Kroner, BL 2005, 'Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection: A nested cross-sectional study', BMC Blood Disorders, vol. 5. https://doi.org/10.1186/1471-2326-5-2

    Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection : A nested cross-sectional study. / Zhang, Mingdong; O'Brien, Thomas R.; Kopp, William C.; Goedert, James J.; Rosenberg, P. S.; Rabkin, C. S.; Engels, E. A.; Whitby, D.; Eyster, M. Elaine; Konkle, B.; Manco-Johnson, M.; DiMichele, D.; Hilgartner, M. W.; Blatt, P.; Aledort, L. M.; Seremetes, S.; Hoots, K.; Angiolillo, A. L.; Luban, N. L.C.; Cohen, A.; Manno, C. S.; Leissinger, C.; White, G. C.; Lederman, M. M.; Purvis, S.; Salkowitz, J.; Kessler, C. M.; Karafoulidou, A.; Mandalaki, T.; Hatzakis, A.; Touloumi, G.; Schramm, W.; Rommel, F.; de Moerloose, P.; Eichinger, S.; Sherman, K. E.; Kroner, B. L.

    In: BMC Blood Disorders, Vol. 5, 18.01.2005.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Lymphocyte subsets in hemophilic patients with hepatitis C virus infection with or without human immunodeficiency virus co-infection

    T2 - A nested cross-sectional study

    AU - Zhang, Mingdong

    AU - O'Brien, Thomas R.

    AU - Kopp, William C.

    AU - Goedert, James J.

    AU - Rosenberg, P. S.

    AU - Rabkin, C. S.

    AU - Engels, E. A.

    AU - Whitby, D.

    AU - Eyster, M. Elaine

    AU - Konkle, B.

    AU - Manco-Johnson, M.

    AU - DiMichele, D.

    AU - Hilgartner, M. W.

    AU - Blatt, P.

    AU - Aledort, L. M.

    AU - Seremetes, S.

    AU - Hoots, K.

    AU - Angiolillo, A. L.

    AU - Luban, N. L.C.

    AU - Cohen, A.

    AU - Manno, C. S.

    AU - Leissinger, C.

    AU - White, G. C.

    AU - Lederman, M. M.

    AU - Purvis, S.

    AU - Salkowitz, J.

    AU - Kessler, C. M.

    AU - Karafoulidou, A.

    AU - Mandalaki, T.

    AU - Hatzakis, A.

    AU - Touloumi, G.

    AU - Schramm, W.

    AU - Rommel, F.

    AU - de Moerloose, P.

    AU - Eichinger, S.

    AU - Sherman, K. E.

    AU - Kroner, B. L.

    PY - 2005/1/18

    Y1 - 2005/1/18

    N2 - Background: With chronic infection, hepatitis C virus (HCV) RNA can be detected in B cells and associated with B-cell disorders, but these are not well defined. Methods: The relationship between HCV infection and lymphocyte subpopulations was evaluated rigorously in 120 asymptomatic hemophilic patients, randomly selected from a prospective cohort study. CD4+ T cells, CD8+ T cells, CD19+ B cells, and CD56+ NK cells were quantified by flow cytometry using cryopreserved peripheral blood mononuclear cells of 24 hemophilic patients in each of five age-matched groups [uninfected; chronic HCV with or without human immunodeficiency virus (HIV); and cleared HCV with or without HIV]. Results: As expected, patients with HIV had significantly reduced CD4+ and increased CD8+ T cells. Irrespective of HIV, patients with chronic HCV infection had approximately 25% fewer CD19+ B cells than those without chronic HCV infection. Conclusions: These data support the hypothesis that asymptomatic patients with chronic HCV infection have an altered B-lymphocyte population.

    AB - Background: With chronic infection, hepatitis C virus (HCV) RNA can be detected in B cells and associated with B-cell disorders, but these are not well defined. Methods: The relationship between HCV infection and lymphocyte subpopulations was evaluated rigorously in 120 asymptomatic hemophilic patients, randomly selected from a prospective cohort study. CD4+ T cells, CD8+ T cells, CD19+ B cells, and CD56+ NK cells were quantified by flow cytometry using cryopreserved peripheral blood mononuclear cells of 24 hemophilic patients in each of five age-matched groups [uninfected; chronic HCV with or without human immunodeficiency virus (HIV); and cleared HCV with or without HIV]. Results: As expected, patients with HIV had significantly reduced CD4+ and increased CD8+ T cells. Irrespective of HIV, patients with chronic HCV infection had approximately 25% fewer CD19+ B cells than those without chronic HCV infection. Conclusions: These data support the hypothesis that asymptomatic patients with chronic HCV infection have an altered B-lymphocyte population.

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    UR - http://www.scopus.com/inward/citedby.url?scp=13644250284&partnerID=8YFLogxK

    U2 - 10.1186/1471-2326-5-2

    DO - 10.1186/1471-2326-5-2

    M3 - Article

    VL - 5

    JO - BMC Hematology

    JF - BMC Hematology

    SN - 1471-2326

    ER -