TY - JOUR
T1 - Mafb and c-Maf Have Prenatal Compensatory and Postnatal Antagonistic Roles in Cortical Interneuron Fate and Function
AU - Pai, Emily Ling Lin
AU - Vogt, Daniel
AU - Clemente-Perez, Alexandra
AU - McKinsey, Gabriel L.
AU - Cho, Frances S.
AU - Hu, Jia Sheng
AU - Wimer, Matt
AU - Paul, Anirban
AU - Fazel Darbandi, Siavash
AU - Pla, Ramon
AU - Nowakowski, Tomasz J.
AU - Goodrich, Lisa V.
AU - Paz, Jeanne T.
AU - Rubenstein, John L.R.
N1 - Funding Information:
We thank Dr. Josh Huang for the mouse P40, single-cell, RNA-seq data for Mafb and c-Maf expression in interneurons. We thank Dr. Mercedes Paredes and Dr. Arturo Alvarez-Buylla for the goat-MCM2 and mouse-Ki67 antibodies. We thank UCSF Mission Bay Nikon Imaging Center for the confocal microscopy service. We thank our lab manager Carol Kim for helping with all the animal care and breeding. This work was supported by the following research grants: Nina Ireland NIMH R01 MH081880 and NIMH R37/R01 MH049428 (to J.L.R.R.); UCSF CTSI 1111111 and Alliance Funds (to D.V.); UCSF neuroscience graduate program (to E.L.-L.P.); National Science Foundation 1650113 (to A.C.-P.); and NINDS 5R00NS078118-04 and 5R01NS096369-02, Department of Defense EP150038, National Science Foundation 1608234, and the Gladstone Institutes (to J.T.P.).
Funding Information:
We thank Dr. Josh Huang for the mouse P40, single-cell, RNA-seq data for Mafb and c-Maf expression in interneurons. We thank Dr. Mercedes Paredes and Dr. Arturo Alvarez-Buylla for the goat-MCM2 and mouse-Ki67 antibodies. We thank UCSF Mission Bay Nikon Imaging Center for the confocal microscopy service. We thank our lab manager Carol Kim for helping with all the animal care and breeding. This work was supported by the following research grants: Nina Ireland NIMH R01 MH081880 and NIMH R37/R01 MH049428 (to J.L.R.R.); UCSF CTSI 1111111 and Alliance Funds (to D.V.); UCSF neuroscience graduate program (to E.L.-L.P.); National Science Foundation 1650113 (to A.C.-P.); and NINDS 5R00NS078118-04 and 5R01NS096369-02 , Department of Defense EP150038 , National Science Foundation 1608234 , and the Gladstone Institutes (to J.T.P.).
Publisher Copyright:
© 2019 UCSF
PY - 2019/1/29
Y1 - 2019/1/29
N2 - Mafb and c-Maf transcription factor (TF) expression is enriched in medial ganglionic eminence (MGE) lineages, beginning in late-secondary progenitors and continuing into mature parvalbumin (PV+) and somatostatin (SST+) interneurons. However, the functions of Maf TFs in MGE development remain to be elucidated. Herein, Mafb and c-Maf were conditionally deleted, alone and together, in the MGE and its lineages. Analyses of Maf mutant mice revealed redundant functions of Mafb and c-Maf in secondary MGE progenitors, where they repress the generation of SST+ cortical and hippocampal interneurons. By contrast, Mafb and c-Maf have distinct roles in postnatal cortical interneuron (CIN) morphological maturation, synaptogenesis, and cortical circuit integration. Thus, Mafb and c-Maf have redundant and opposing functions at different steps in CIN development.
AB - Mafb and c-Maf transcription factor (TF) expression is enriched in medial ganglionic eminence (MGE) lineages, beginning in late-secondary progenitors and continuing into mature parvalbumin (PV+) and somatostatin (SST+) interneurons. However, the functions of Maf TFs in MGE development remain to be elucidated. Herein, Mafb and c-Maf were conditionally deleted, alone and together, in the MGE and its lineages. Analyses of Maf mutant mice revealed redundant functions of Mafb and c-Maf in secondary MGE progenitors, where they repress the generation of SST+ cortical and hippocampal interneurons. By contrast, Mafb and c-Maf have distinct roles in postnatal cortical interneuron (CIN) morphological maturation, synaptogenesis, and cortical circuit integration. Thus, Mafb and c-Maf have redundant and opposing functions at different steps in CIN development.
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U2 - 10.1016/j.celrep.2019.01.031
DO - 10.1016/j.celrep.2019.01.031
M3 - Article
C2 - 30699346
AN - SCOPUS:85060326599
SN - 2211-1247
VL - 26
SP - 1157-1173.e5
JO - Cell Reports
JF - Cell Reports
IS - 5
ER -