Maghemite, silver, ceragenin conjugate particles for selective binding and contrast of bacteria

Mark A. Hoppens, Zaanan E.W. Wheeler, Ammar T. Qureshi, Katie Hogan, Ashleigh Wright, George G. Stanley, David Young, Paul Savage, Daniel J. Hayes

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

New synthesis techniques are providing increasing control over many inorganic nanoparticle characteristics, facilitating the creation of new multifunctional theranostics. This report proposes the synthesis and testing of a combination nanoparticle comprised of a maghemite core for enhanced T2 MRI contrast diagnostics, a colloidal silver shell acting as an antimicrobial and therapeutic vehicle, and a ceragenin (CSA-124) surfactant providing microbial adhesion. A polyacrylic acid functionalized maghemite nanoparticle is synthesized by a high temperature organic phase reduction followed by thiol functionalization and gold cluster seeding. A silver shell is formed through AgNO3 reduction, and an oriented monolayer of the thiolated ceragenin, is bound through a self-assembly process. The process and products are characterized throughout synthesis through TEM, DLS, FT-IR, UV-Vis, ICP-OES, HPLC-ESI-TOF-MS, DC magnetization and susceptibility, X-ray diffraction, and in vitro MRI. Synthesized Diagnostic Antimicrobial Nanoparticles (DANs) were found to have a spherical morphology with a diameter of 32.47±1.83nm, hydrodynamic diameter of 53.05±1.20nm, maximum magnetic moment of 12emu/g NP (54emu/g Fe) with little variation due to temperature, and are predominantly paramagnetic. In vitro MRI studies show that DANs contrast well at concentrations as low as 9ppm, and successfully adhere to Staphylococcus aureus. DAN MIC was determined to be approximately 12ppm and 24ppm against S. aureus and Escherichia coli respectively.

Original languageEnglish (US)
Pages (from-to)167-174
Number of pages8
JournalJournal of Colloid And Interface Science
Volume413
DOIs
StatePublished - Jan 1 2014

Fingerprint

Silver
Bacteria
Nanoparticles
Magnetic resonance imaging
carbopol 940
Magnetic moments
Sulfhydryl Compounds
Surface-Active Agents
Gold
Self assembly
Escherichia coli
ferric oxide
Monolayers
Magnetization
Surface active agents
Adhesion
Hydrodynamics
Transmission electron microscopy
X ray diffraction
Temperature

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Surfaces, Coatings and Films
  • Colloid and Surface Chemistry

Cite this

Hoppens, Mark A. ; Wheeler, Zaanan E.W. ; Qureshi, Ammar T. ; Hogan, Katie ; Wright, Ashleigh ; Stanley, George G. ; Young, David ; Savage, Paul ; Hayes, Daniel J. / Maghemite, silver, ceragenin conjugate particles for selective binding and contrast of bacteria. In: Journal of Colloid And Interface Science. 2014 ; Vol. 413. pp. 167-174.
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Hoppens, MA, Wheeler, ZEW, Qureshi, AT, Hogan, K, Wright, A, Stanley, GG, Young, D, Savage, P & Hayes, DJ 2014, 'Maghemite, silver, ceragenin conjugate particles for selective binding and contrast of bacteria', Journal of Colloid And Interface Science, vol. 413, pp. 167-174. https://doi.org/10.1016/j.jcis.2013.09.016

Maghemite, silver, ceragenin conjugate particles for selective binding and contrast of bacteria. / Hoppens, Mark A.; Wheeler, Zaanan E.W.; Qureshi, Ammar T.; Hogan, Katie; Wright, Ashleigh; Stanley, George G.; Young, David; Savage, Paul; Hayes, Daniel J.

In: Journal of Colloid And Interface Science, Vol. 413, 01.01.2014, p. 167-174.

Research output: Contribution to journalArticle

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