TY - GEN
T1 - Magnetic bio-nanobeads and nanoelectrode based impedance biosensor for detection of avian influenza virus
AU - Wang, Ronghui
AU - Li, Yanbin
AU - Mao, Xiaole
AU - Huang, Tony
AU - Lu, Huaguang
PY - 2010/12/1
Y1 - 2010/12/1
N2 - A novel impedance biosensor was developed based on the combination of a bio-nanobead separation/concentration procedure and an interdigitated array nanoeletrode and was demonstrated for sensitive and rapid detection of H5 subtype of avian influenza virus (AIV). Magnetic nanobeads with a diameter of 30 nm were coated with H5 subtype-specific monoclonal antibodies to selectively capture the target virus. An interdigitated array nanoeletrode was designed and fabricated for impedance measurement. Changes in the impedance of the antibody coated nanobead-virus complex was measured and correlated to the presence of H5 AIV (e.g., H5N1). The nanobead and nanoeletrode based impedance biosensor was able to detect AIV H5N1 at titer of 0.0128 HA unit/50 μl. Equivalent circuit analysis indicated that the solution resistance was responsible for the impedance change due to the presence of target virus.
AB - A novel impedance biosensor was developed based on the combination of a bio-nanobead separation/concentration procedure and an interdigitated array nanoeletrode and was demonstrated for sensitive and rapid detection of H5 subtype of avian influenza virus (AIV). Magnetic nanobeads with a diameter of 30 nm were coated with H5 subtype-specific monoclonal antibodies to selectively capture the target virus. An interdigitated array nanoeletrode was designed and fabricated for impedance measurement. Changes in the impedance of the antibody coated nanobead-virus complex was measured and correlated to the presence of H5 AIV (e.g., H5N1). The nanobead and nanoeletrode based impedance biosensor was able to detect AIV H5N1 at titer of 0.0128 HA unit/50 μl. Equivalent circuit analysis indicated that the solution resistance was responsible for the impedance change due to the presence of target virus.
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U2 - 10.1109/NANOMED.2010.5749837
DO - 10.1109/NANOMED.2010.5749837
M3 - Conference contribution
AN - SCOPUS:79956008697
SN - 9781612841533
T3 - 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
SP - 214
EP - 217
BT - 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
T2 - 4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
Y2 - 5 December 2010 through 9 December 2010
ER -