Magnetic bio-nanobeads and nanoelectrode based impedance biosensor for detection of avian influenza virus

Ronghui Wang, Yanbin Li, Xiaole Mao, Tony Huang, Huaguang Lu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A novel impedance biosensor was developed based on the combination of a bio-nanobead separation/concentration procedure and an interdigitated array nanoeletrode and was demonstrated for sensitive and rapid detection of H5 subtype of avian influenza virus (AIV). Magnetic nanobeads with a diameter of 30 nm were coated with H5 subtype-specific monoclonal antibodies to selectively capture the target virus. An interdigitated array nanoeletrode was designed and fabricated for impedance measurement. Changes in the impedance of the antibody coated nanobead-virus complex was measured and correlated to the presence of H5 AIV (e.g., H5N1). The nanobead and nanoeletrode based impedance biosensor was able to detect AIV H5N1 at titer of 0.0128 HA unit/50 μl. Equivalent circuit analysis indicated that the solution resistance was responsible for the impedance change due to the presence of target virus.

Original languageEnglish (US)
Title of host publication2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
Pages214-217
Number of pages4
DOIs
StatePublished - Dec 1 2010
Event4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010 - Hong Kong/Macau, China
Duration: Dec 5 2010Dec 9 2010

Publication series

Name2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010

Other

Other4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
CountryChina
CityHong Kong/Macau
Period12/5/1012/9/10

Fingerprint

Influenza in Birds
Biosensing Techniques
Orthomyxoviridae
Electric Impedance
Viruses
Monoclonal Antibodies
Antibodies

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology

Cite this

Wang, R., Li, Y., Mao, X., Huang, T., & Lu, H. (2010). Magnetic bio-nanobeads and nanoelectrode based impedance biosensor for detection of avian influenza virus. In 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010 (pp. 214-217). [5749837] (2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010). https://doi.org/10.1109/NANOMED.2010.5749837
Wang, Ronghui ; Li, Yanbin ; Mao, Xiaole ; Huang, Tony ; Lu, Huaguang. / Magnetic bio-nanobeads and nanoelectrode based impedance biosensor for detection of avian influenza virus. 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010. 2010. pp. 214-217 (2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010).
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abstract = "A novel impedance biosensor was developed based on the combination of a bio-nanobead separation/concentration procedure and an interdigitated array nanoeletrode and was demonstrated for sensitive and rapid detection of H5 subtype of avian influenza virus (AIV). Magnetic nanobeads with a diameter of 30 nm were coated with H5 subtype-specific monoclonal antibodies to selectively capture the target virus. An interdigitated array nanoeletrode was designed and fabricated for impedance measurement. Changes in the impedance of the antibody coated nanobead-virus complex was measured and correlated to the presence of H5 AIV (e.g., H5N1). The nanobead and nanoeletrode based impedance biosensor was able to detect AIV H5N1 at titer of 0.0128 HA unit/50 μl. Equivalent circuit analysis indicated that the solution resistance was responsible for the impedance change due to the presence of target virus.",
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Wang, R, Li, Y, Mao, X, Huang, T & Lu, H 2010, Magnetic bio-nanobeads and nanoelectrode based impedance biosensor for detection of avian influenza virus. in 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010., 5749837, 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010, pp. 214-217, 4th IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010, Hong Kong/Macau, China, 12/5/10. https://doi.org/10.1109/NANOMED.2010.5749837

Magnetic bio-nanobeads and nanoelectrode based impedance biosensor for detection of avian influenza virus. / Wang, Ronghui; Li, Yanbin; Mao, Xiaole; Huang, Tony; Lu, Huaguang.

2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010. 2010. p. 214-217 5749837 (2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Wang R, Li Y, Mao X, Huang T, Lu H. Magnetic bio-nanobeads and nanoelectrode based impedance biosensor for detection of avian influenza virus. In 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010. 2010. p. 214-217. 5749837. (2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010). https://doi.org/10.1109/NANOMED.2010.5749837