Magnetic resonance imaging of acute injury in rats and the effects of buprenorphine on limb volume

Nicole M. McBrier, Thomas Neuberger, Craig R. Denegar, Neil Sharkey, Andrew G. Webb

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

The purposes of this study were to determine 1) whether magnetic resonance imaging (MRI)-based T2 mapping and measurements of limb volume can differentiate injured and uninjured tissue after blunt trauma to rat hindlimbs and 2) whether administration of buprenorphine influences these assessments. Male Wistar rats (age, 3 to 4 mo) underwent blunt contusion injury to the posterior aspect of the hindlimb; MRI was conducted at 6,12,24,48,72, and 96 h after injury. The imaging results showed that administration of buprenorphine had no effect on the T2 value {area under the receiver operating characteristic [ROC] curve: with drug, 0.869 [95% confidence interval (CI), 0.78 to 0.96]; without drug, 0.809 [95% CI, 0.72 to 0.90]} but did influence limb volume [area under the ROC curve; without drug, 0.954 (95% CI, 0.92 to 0.99); with drug, 0.713 (95% CI, 0.61 to 0.82)]. When using MRI to determine the extent of injury or to track injury over time, calculated limb volumes may lose sensitivity to detect injury, due to the intrinsic increase in volume from morphine-derived drugs. During administration of morphine derivatives, T2 maps may provide more accurate assessments of muscle tissue injury both initially after injury and over time.

Original languageEnglish (US)
Pages (from-to)147-151
Number of pages5
JournalJournal of the American Association for Laboratory Animal Science
Volume48
Issue number2
StatePublished - Mar 1 2009

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Fingerprint Dive into the research topics of 'Magnetic resonance imaging of acute injury in rats and the effects of buprenorphine on limb volume'. Together they form a unique fingerprint.

  • Cite this