Magnetic resonance studies of the anomeric distribution and manganese binding properties of fructose phosphates

Stephen Benkovic, James L. Engle, Albert S. Mildvan

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The anomeric distributions of the furanose forms of fructose-6-P and fructose-1,6-diP have been found by 13C NMR to be 20 ± 4% α and 80 ± 10% β. The dissociation constants of the Mn complexes of fructose-1,6-diP and of a configurationally stable cis-1,6-diP analog are similar to those of various Mn-monophosphate complexes and are therefore inconsistent with chelation of the Mn by the cis-diphosphoryl moieties. Thus the affinity for metal is an unsatisfactory method for diagnosis of anomeric equilibria.

Original languageEnglish (US)
Pages (from-to)852-858
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume47
Issue number4
DOIs
StatePublished - May 26 1972

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Magnetic resonance
Manganese
Fructose
Magnetic Resonance Spectroscopy
Phosphates
Chelation
Metals
Nuclear magnetic resonance

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "The anomeric distributions of the furanose forms of fructose-6-P and fructose-1,6-diP have been found by 13C NMR to be 20 ± 4{\%} α and 80 ± 10{\%} β. The dissociation constants of the Mn complexes of fructose-1,6-diP and of a configurationally stable cis-1,6-diP analog are similar to those of various Mn-monophosphate complexes and are therefore inconsistent with chelation of the Mn by the cis-diphosphoryl moieties. Thus the affinity for metal is an unsatisfactory method for diagnosis of anomeric equilibria.",
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Magnetic resonance studies of the anomeric distribution and manganese binding properties of fructose phosphates. / Benkovic, Stephen; Engle, James L.; Mildvan, Albert S.

In: Biochemical and Biophysical Research Communications, Vol. 47, No. 4, 26.05.1972, p. 852-858.

Research output: Contribution to journalArticle

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AB - The anomeric distributions of the furanose forms of fructose-6-P and fructose-1,6-diP have been found by 13C NMR to be 20 ± 4% α and 80 ± 10% β. The dissociation constants of the Mn complexes of fructose-1,6-diP and of a configurationally stable cis-1,6-diP analog are similar to those of various Mn-monophosphate complexes and are therefore inconsistent with chelation of the Mn by the cis-diphosphoryl moieties. Thus the affinity for metal is an unsatisfactory method for diagnosis of anomeric equilibria.

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