Major histocompatibility complex of the mole-rat - II. Restriction fragment polymorphism

D. Nižetić, F. Figueroa, E. Nevo, J. Klein

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The major histocompatibility complex (Mhc) is a group of loci coding for lymphocyte membrane glycoproteins that provide the context for the recognition of foreign antigens in the initial phase of the immune response. The complex contains a large number of loci, some of which are highly polymorphic. The complexity and polymorphism pose a number of questions concerning the evolution of the Mhc. In an attempt to answer some of these questions, we have begun to study the Mhc of the mole-rat, Spalax ehrenbergi, a rodent representing a complex of sibling species occupying ecologically and geographically clearly delineated regions within the borders of Israel. In an earlier publication we identified the Spalax major histocompatibility (Smh) complex serologically and biochemically. Here, we analyze the Smh by Southern blotting of DNA fragments produced by restriction enzyme digestion. The fragments were hybridized to mouse probes specific for class I, class II, and C4 genes. The analysis has revealed that the Smh complex contains as many class I genes as the mouse does and that these genes are polymorphic. The number of class II genes could not be determined with certainty, but it is probably not greater than in the mouse. Polymorphism was also detected at the loci coding for the complement component 4 (C4), which are probably closely linked to the Smh complex. The polymorphism of mole-rat class I loci contrasts with the reported monomorphism of these loci in the Syrian hamster. Since the mole-rat leads a solitary, subterranean life, as the Syrian hamster does, ecology cannot be an explanation for the lack of class I polymorphism in the latter species.

Original languageEnglish (US)
Pages (from-to)55-67
Number of pages13
JournalImmunogenetics
Volume22
Issue number1
DOIs
StatePublished - Jul 1985

Fingerprint

Mole Rats
Spalax
Major Histocompatibility Complex
Mesocricetus
MHC Class I Genes
Gene Components
Complement C4
MHC Class II Genes
Histocompatibility
Membrane Glycoproteins
Israel
Southern Blotting
Ecology
Digestion
Rodentia
Lymphocytes
Antigens
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics

Cite this

Nižetić, D. ; Figueroa, F. ; Nevo, E. ; Klein, J. / Major histocompatibility complex of the mole-rat - II. Restriction fragment polymorphism. In: Immunogenetics. 1985 ; Vol. 22, No. 1. pp. 55-67.
@article{fff206597b534ea38a9bc8a9bd521505,
title = "Major histocompatibility complex of the mole-rat - II. Restriction fragment polymorphism",
abstract = "The major histocompatibility complex (Mhc) is a group of loci coding for lymphocyte membrane glycoproteins that provide the context for the recognition of foreign antigens in the initial phase of the immune response. The complex contains a large number of loci, some of which are highly polymorphic. The complexity and polymorphism pose a number of questions concerning the evolution of the Mhc. In an attempt to answer some of these questions, we have begun to study the Mhc of the mole-rat, Spalax ehrenbergi, a rodent representing a complex of sibling species occupying ecologically and geographically clearly delineated regions within the borders of Israel. In an earlier publication we identified the Spalax major histocompatibility (Smh) complex serologically and biochemically. Here, we analyze the Smh by Southern blotting of DNA fragments produced by restriction enzyme digestion. The fragments were hybridized to mouse probes specific for class I, class II, and C4 genes. The analysis has revealed that the Smh complex contains as many class I genes as the mouse does and that these genes are polymorphic. The number of class II genes could not be determined with certainty, but it is probably not greater than in the mouse. Polymorphism was also detected at the loci coding for the complement component 4 (C4), which are probably closely linked to the Smh complex. The polymorphism of mole-rat class I loci contrasts with the reported monomorphism of these loci in the Syrian hamster. Since the mole-rat leads a solitary, subterranean life, as the Syrian hamster does, ecology cannot be an explanation for the lack of class I polymorphism in the latter species.",
author = "D. Nižetić and F. Figueroa and E. Nevo and J. Klein",
year = "1985",
month = "7",
doi = "10.1007/BF00430594",
language = "English (US)",
volume = "22",
pages = "55--67",
journal = "Immunogenetics",
issn = "0093-7711",
publisher = "Springer Verlag",
number = "1",

}

Major histocompatibility complex of the mole-rat - II. Restriction fragment polymorphism. / Nižetić, D.; Figueroa, F.; Nevo, E.; Klein, J.

In: Immunogenetics, Vol. 22, No. 1, 07.1985, p. 55-67.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Major histocompatibility complex of the mole-rat - II. Restriction fragment polymorphism

AU - Nižetić, D.

AU - Figueroa, F.

AU - Nevo, E.

AU - Klein, J.

PY - 1985/7

Y1 - 1985/7

N2 - The major histocompatibility complex (Mhc) is a group of loci coding for lymphocyte membrane glycoproteins that provide the context for the recognition of foreign antigens in the initial phase of the immune response. The complex contains a large number of loci, some of which are highly polymorphic. The complexity and polymorphism pose a number of questions concerning the evolution of the Mhc. In an attempt to answer some of these questions, we have begun to study the Mhc of the mole-rat, Spalax ehrenbergi, a rodent representing a complex of sibling species occupying ecologically and geographically clearly delineated regions within the borders of Israel. In an earlier publication we identified the Spalax major histocompatibility (Smh) complex serologically and biochemically. Here, we analyze the Smh by Southern blotting of DNA fragments produced by restriction enzyme digestion. The fragments were hybridized to mouse probes specific for class I, class II, and C4 genes. The analysis has revealed that the Smh complex contains as many class I genes as the mouse does and that these genes are polymorphic. The number of class II genes could not be determined with certainty, but it is probably not greater than in the mouse. Polymorphism was also detected at the loci coding for the complement component 4 (C4), which are probably closely linked to the Smh complex. The polymorphism of mole-rat class I loci contrasts with the reported monomorphism of these loci in the Syrian hamster. Since the mole-rat leads a solitary, subterranean life, as the Syrian hamster does, ecology cannot be an explanation for the lack of class I polymorphism in the latter species.

AB - The major histocompatibility complex (Mhc) is a group of loci coding for lymphocyte membrane glycoproteins that provide the context for the recognition of foreign antigens in the initial phase of the immune response. The complex contains a large number of loci, some of which are highly polymorphic. The complexity and polymorphism pose a number of questions concerning the evolution of the Mhc. In an attempt to answer some of these questions, we have begun to study the Mhc of the mole-rat, Spalax ehrenbergi, a rodent representing a complex of sibling species occupying ecologically and geographically clearly delineated regions within the borders of Israel. In an earlier publication we identified the Spalax major histocompatibility (Smh) complex serologically and biochemically. Here, we analyze the Smh by Southern blotting of DNA fragments produced by restriction enzyme digestion. The fragments were hybridized to mouse probes specific for class I, class II, and C4 genes. The analysis has revealed that the Smh complex contains as many class I genes as the mouse does and that these genes are polymorphic. The number of class II genes could not be determined with certainty, but it is probably not greater than in the mouse. Polymorphism was also detected at the loci coding for the complement component 4 (C4), which are probably closely linked to the Smh complex. The polymorphism of mole-rat class I loci contrasts with the reported monomorphism of these loci in the Syrian hamster. Since the mole-rat leads a solitary, subterranean life, as the Syrian hamster does, ecology cannot be an explanation for the lack of class I polymorphism in the latter species.

UR - http://www.scopus.com/inward/record.url?scp=0021931516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021931516&partnerID=8YFLogxK

U2 - 10.1007/BF00430594

DO - 10.1007/BF00430594

M3 - Article

C2 - 2991131

AN - SCOPUS:0021931516

VL - 22

SP - 55

EP - 67

JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

IS - 1

ER -