Male-Produced Pheromone in the European Woodwasp, Sirex noctilio

Miriam F. Cooperband, Katalin Böröczky, Ashley Hartness, Tappey H. Jones, Kelley E. Zylstra, James Homer Tumlinson, III, Victor C. Mastro

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A male-produced pheromone that attracts both males and females was identified for the European woodwasp, Sirex noctilio, a serious pest of pine trees. Males displayed excitatory behaviors when placed in groups, and were attracted to the odors from males that were 2-5-d-old, but not to odors from males that were 0-1-d-old. An unsaturated short-chain alcohol, (Z)-3-decen-1-ol, was discovered in samples collected on SuperQ filters over groups of males and identified by using micro-derivatization reactions and gas chromatography coupled with mass spectrometry (GC-MS). The compound was not detected in volatile samples from females. Gas chromatography coupled electroantennographic detection (GC-EAD) of antennae from males exposed to male headspace odors produced strong antennal responses to the main peak of (Z)-3-decen-1-ol, as well as to an unknown minor component that had a similar retention time. Antennae from both males and females responded to synthetic (Z)-3-decen-1-ol. Several different synthetic candidates for the GC-EAD active minor components were selected based on GC-MS and GC-EAD responses to male headspace collections. These synthetic compounds were tested for antennal activity using GC-EAD, and those that produced strong responses were blended with the major component and tested for male attraction in the Y-tube olfactometer at different concentrations and ratios. Males tested in the Y-tube olfactometer were attracted to a synthetic blend of (Z)-3-decen-1-ol and (Z)-4-decen-1-ol at a ratio of 100:1. Whereas the addition of some suspected minor compounds reduced attraction, the addition of a third compound found in male emanations that produced strong male antennal responses, (E,E)-2,4-decadienal (at a ratio of 100:1:1), resulted in attraction of both males (Y-tube and wind tunnel) and females (wind tunnel).

Original languageEnglish (US)
Pages (from-to)52-62
Number of pages11
JournalJournal of Chemical Ecology
Volume38
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Sirex noctilio
Pheromones
pheromone
Gas chromatography
pheromones
Odors
electroantennography
gas chromatography
Gas Chromatography
Wind tunnels
Mass spectrometry
Antennas
odor
olfactometers
wind tunnels
odors
wind tunnel
headspace analysis
antennae
Alcohols

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

Cite this

Cooperband, M. F., Böröczky, K., Hartness, A., Jones, T. H., Zylstra, K. E., Tumlinson, III, J. H., & Mastro, V. C. (2012). Male-Produced Pheromone in the European Woodwasp, Sirex noctilio. Journal of Chemical Ecology, 38(1), 52-62. https://doi.org/10.1007/s10886-012-0060-7
Cooperband, Miriam F. ; Böröczky, Katalin ; Hartness, Ashley ; Jones, Tappey H. ; Zylstra, Kelley E. ; Tumlinson, III, James Homer ; Mastro, Victor C. / Male-Produced Pheromone in the European Woodwasp, Sirex noctilio. In: Journal of Chemical Ecology. 2012 ; Vol. 38, No. 1. pp. 52-62.
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Cooperband, MF, Böröczky, K, Hartness, A, Jones, TH, Zylstra, KE, Tumlinson, III, JH & Mastro, VC 2012, 'Male-Produced Pheromone in the European Woodwasp, Sirex noctilio', Journal of Chemical Ecology, vol. 38, no. 1, pp. 52-62. https://doi.org/10.1007/s10886-012-0060-7

Male-Produced Pheromone in the European Woodwasp, Sirex noctilio. / Cooperband, Miriam F.; Böröczky, Katalin; Hartness, Ashley; Jones, Tappey H.; Zylstra, Kelley E.; Tumlinson, III, James Homer; Mastro, Victor C.

In: Journal of Chemical Ecology, Vol. 38, No. 1, 01.01.2012, p. 52-62.

Research output: Contribution to journalArticle

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AU - Böröczky, Katalin

AU - Hartness, Ashley

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