Mammalian PSP24s (α and β isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems

Yuka Imamura, Kazuhiko Kume, Takashi Izumi, Takao Shimizu

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Xenopus PSP24 (xPSP24) is a G-protein-coupled receptor which was originally identified as a functional lysophosphatidic acid (LPA) receptor. We obtained two different types (α and β) of mammalian homologues of xPSP24 and found that these receptors are highly expressed in the brain (Kawasawa et al., Biochem. Biophys. Res. Commun. 276, 952-956, 2000). These receptor did not respond to LPA by GTPγS binding assays, while Edg2 or Edg4 showed responses to LPA under the same assay conditions. Furthermore, a sensitive reporter gene assay using PC12 cells with serum response element promoter failed to detect any response of mammalian PSP24s to various concentrations of LPA. Thus, unlike xPSP24, we conclude that PSP24s are not functional LPA receptors in mammalian systems. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)957-964
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume276
Issue number3
DOIs
StatePublished - Oct 5 2000

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Lysophosphatidic Acid Receptors
Assays
Protein Isoforms
Xenopus
Serum Response Element
PC12 Cells
G-Protein-Coupled Receptors
Reporter Genes
Brain
Genes
lysophosphatidic acid

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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Mammalian PSP24s (α and β isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems. / Imamura, Yuka; Kume, Kazuhiko; Izumi, Takashi; Shimizu, Takao.

In: Biochemical and Biophysical Research Communications, Vol. 276, No. 3, 05.10.2000, p. 957-964.

Research output: Contribution to journalArticle

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T1 - Mammalian PSP24s (α and β isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems

AU - Imamura, Yuka

AU - Kume, Kazuhiko

AU - Izumi, Takashi

AU - Shimizu, Takao

PY - 2000/10/5

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N2 - Xenopus PSP24 (xPSP24) is a G-protein-coupled receptor which was originally identified as a functional lysophosphatidic acid (LPA) receptor. We obtained two different types (α and β) of mammalian homologues of xPSP24 and found that these receptors are highly expressed in the brain (Kawasawa et al., Biochem. Biophys. Res. Commun. 276, 952-956, 2000). These receptor did not respond to LPA by GTPγS binding assays, while Edg2 or Edg4 showed responses to LPA under the same assay conditions. Furthermore, a sensitive reporter gene assay using PC12 cells with serum response element promoter failed to detect any response of mammalian PSP24s to various concentrations of LPA. Thus, unlike xPSP24, we conclude that PSP24s are not functional LPA receptors in mammalian systems. (C) 2000 Academic Press.

AB - Xenopus PSP24 (xPSP24) is a G-protein-coupled receptor which was originally identified as a functional lysophosphatidic acid (LPA) receptor. We obtained two different types (α and β) of mammalian homologues of xPSP24 and found that these receptors are highly expressed in the brain (Kawasawa et al., Biochem. Biophys. Res. Commun. 276, 952-956, 2000). These receptor did not respond to LPA by GTPγS binding assays, while Edg2 or Edg4 showed responses to LPA under the same assay conditions. Furthermore, a sensitive reporter gene assay using PC12 cells with serum response element promoter failed to detect any response of mammalian PSP24s to various concentrations of LPA. Thus, unlike xPSP24, we conclude that PSP24s are not functional LPA receptors in mammalian systems. (C) 2000 Academic Press.

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