Abstract
Xenopus PSP24 (xPSP24) is a G-protein-coupled receptor which was originally identified as a functional lysophosphatidic acid (LPA) receptor. We obtained two different types (α and β) of mammalian homologues of xPSP24 and found that these receptors are highly expressed in the brain (Kawasawa et al., Biochem. Biophys. Res. Commun. 276, 952-956, 2000). These receptor did not respond to LPA by GTPγS binding assays, while Edg2 or Edg4 showed responses to LPA under the same assay conditions. Furthermore, a sensitive reporter gene assay using PC12 cells with serum response element promoter failed to detect any response of mammalian PSP24s to various concentrations of LPA. Thus, unlike xPSP24, we conclude that PSP24s are not functional LPA receptors in mammalian systems. (C) 2000 Academic Press.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 957-964 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 276 |
| Issue number | 3 |
| DOIs | |
| State | Published - Oct 5 2000 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology
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Mammalian PSP24s (α and β isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems. / Imamura, Yuka; Kume, Kazuhiko; Izumi, Takashi; Shimizu, Takao.
In: Biochemical and Biophysical Research Communications, Vol. 276, No. 3, 05.10.2000, p. 957-964.Research output: Contribution to journal › Article
TY - JOUR
T1 - Mammalian PSP24s (α and β isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems
AU - Imamura, Yuka
AU - Kume, Kazuhiko
AU - Izumi, Takashi
AU - Shimizu, Takao
PY - 2000/10/5
Y1 - 2000/10/5
N2 - Xenopus PSP24 (xPSP24) is a G-protein-coupled receptor which was originally identified as a functional lysophosphatidic acid (LPA) receptor. We obtained two different types (α and β) of mammalian homologues of xPSP24 and found that these receptors are highly expressed in the brain (Kawasawa et al., Biochem. Biophys. Res. Commun. 276, 952-956, 2000). These receptor did not respond to LPA by GTPγS binding assays, while Edg2 or Edg4 showed responses to LPA under the same assay conditions. Furthermore, a sensitive reporter gene assay using PC12 cells with serum response element promoter failed to detect any response of mammalian PSP24s to various concentrations of LPA. Thus, unlike xPSP24, we conclude that PSP24s are not functional LPA receptors in mammalian systems. (C) 2000 Academic Press.
AB - Xenopus PSP24 (xPSP24) is a G-protein-coupled receptor which was originally identified as a functional lysophosphatidic acid (LPA) receptor. We obtained two different types (α and β) of mammalian homologues of xPSP24 and found that these receptors are highly expressed in the brain (Kawasawa et al., Biochem. Biophys. Res. Commun. 276, 952-956, 2000). These receptor did not respond to LPA by GTPγS binding assays, while Edg2 or Edg4 showed responses to LPA under the same assay conditions. Furthermore, a sensitive reporter gene assay using PC12 cells with serum response element promoter failed to detect any response of mammalian PSP24s to various concentrations of LPA. Thus, unlike xPSP24, we conclude that PSP24s are not functional LPA receptors in mammalian systems. (C) 2000 Academic Press.
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U2 - 10.1006/bbrc.2000.3570
DO - 10.1006/bbrc.2000.3570
M3 - Article
VL - 276
SP - 957
EP - 964
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -