TY - JOUR
T1 - Mammalian PSP24s (α and β isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems
AU - Kawasawa, Yuka
AU - Kume, Kazuhiko
AU - Izumi, Takashi
AU - Shimizu, Takao
N1 - Funding Information:
We are grateful to Drs. J. Chun, N. Fukushima, and Y. Kimura at the University of California, San Diego, for the generous supply of B103 and RH7777 cells, and Drs. T. Naito and K. Tomura at Japan Tabacco Inc. for collaboration of SRE reporter gene assays. We also thank Dr. D. Wang at the University of Tokyo for comments for the manuscript. This work was supported in part by a Grant-in-Aid from Ministry of Education, Science, Sports, and Culture of Japan.
PY - 2000/10/5
Y1 - 2000/10/5
N2 - Xenopus PSP24 (xPSP24) is a G-protein-coupled receptor which was originally identified as a functional lysophosphatidic acid (LPA) receptor. We obtained two different types (α and β) of mammalian homologues of xPSP24 and found that these receptors are highly expressed in the brain (Kawasawa et al., Biochem. Biophys. Res. Commun. 276, 952-956, 2000). These receptor did not respond to LPA by GTPγS binding assays, while Edg2 or Edg4 showed responses to LPA under the same assay conditions. Furthermore, a sensitive reporter gene assay using PC12 cells with serum response element promoter failed to detect any response of mammalian PSP24s to various concentrations of LPA. Thus, unlike xPSP24, we conclude that PSP24s are not functional LPA receptors in mammalian systems. (C) 2000 Academic Press.
AB - Xenopus PSP24 (xPSP24) is a G-protein-coupled receptor which was originally identified as a functional lysophosphatidic acid (LPA) receptor. We obtained two different types (α and β) of mammalian homologues of xPSP24 and found that these receptors are highly expressed in the brain (Kawasawa et al., Biochem. Biophys. Res. Commun. 276, 952-956, 2000). These receptor did not respond to LPA by GTPγS binding assays, while Edg2 or Edg4 showed responses to LPA under the same assay conditions. Furthermore, a sensitive reporter gene assay using PC12 cells with serum response element promoter failed to detect any response of mammalian PSP24s to various concentrations of LPA. Thus, unlike xPSP24, we conclude that PSP24s are not functional LPA receptors in mammalian systems. (C) 2000 Academic Press.
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U2 - 10.1006/bbrc.2000.3570
DO - 10.1006/bbrc.2000.3570
M3 - Article
C2 - 11027575
AN - SCOPUS:0034610001
VL - 276
SP - 957
EP - 964
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -