Mammalian PSP24s (α and β isoforms) are not responsive to lysophosphatidic acid in mammalian expression systems

Yuka Kawasawa, Kazuhiko Kume, Takashi Izumi, Takao Shimizu

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Xenopus PSP24 (xPSP24) is a G-protein-coupled receptor which was originally identified as a functional lysophosphatidic acid (LPA) receptor. We obtained two different types (α and β) of mammalian homologues of xPSP24 and found that these receptors are highly expressed in the brain (Kawasawa et al., Biochem. Biophys. Res. Commun. 276, 952-956, 2000). These receptor did not respond to LPA by GTPγS binding assays, while Edg2 or Edg4 showed responses to LPA under the same assay conditions. Furthermore, a sensitive reporter gene assay using PC12 cells with serum response element promoter failed to detect any response of mammalian PSP24s to various concentrations of LPA. Thus, unlike xPSP24, we conclude that PSP24s are not functional LPA receptors in mammalian systems. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)957-964
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume276
Issue number3
DOIs
StatePublished - Oct 5 2000

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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