Manipulation of lipid bilayer membranes in solution using laser tweezers and microsphere 'handles'

Christine Dolan Keating, Terrence G. D'Onofrio, Anat Hatzor, Amy S. Whelpley, Michael J. Natan, Paul S. Weiss

Research output: Contribution to journalConference article

3 Citations (Scopus)

Abstract

The local structure of biological membranes is critically important to membrane function. Regions of very high positive and negative curvature are found in the membranes of many cells, and rapid changes in membrane curvature are integral parts of many cell activities (e.g. endo/exocytosis, cell crawling, cell division). Our goal is to understand the effects of changes in local membrane structure on membrane properties. Optical tweezers are used to control the local structure of the lipid bilayer by controlling the curvature of the membrane. We use giant ('cell-sized'), thin-walled vesicles as our membrane models. Optically trapped latex microspheres are used to deform the liposome bilayer, forming large areas of altered membrane curvature. In contrast to literature reports in which 514.5 nm light was used in optical trapping, we have not observed adhesion of uncoated latex microspheres to liposome vesicles, nor have we observed signs of rapidly increased osmotic pressure within irradiated vesicles. This indicates that the longer wavelength used in our studies (647.1 nm) is less damaging to biological membranes. Furthermore, optical trapping of vesicles with coexisting gel and fluid phase lipids did not lead to gross changes in domain structure, which would be expected upon laser-induced heating of the membrane.

Original languageEnglish (US)
Pages (from-to)18-26
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3924
StatePublished - Jan 1 2000
EventMolecular Imaging: Reporters, Dyes, Markers, and Instrumentation - San Jose, CA, USA
Duration: Jan 23 2000Jan 24 2000

Fingerprint

Lipid Bilayer
Lipid bilayers
Microspheres
Membrane Lipids
lipids
Manipulation
manipulators
Membrane
Laser
membranes
Membranes
Lasers
lasers
Vesicles
Biological membranes
curvature
Liposomes
Latex
Latexes
Optical Trapping

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Keating, Christine Dolan ; D'Onofrio, Terrence G. ; Hatzor, Anat ; Whelpley, Amy S. ; Natan, Michael J. ; Weiss, Paul S. / Manipulation of lipid bilayer membranes in solution using laser tweezers and microsphere 'handles'. In: Proceedings of SPIE - The International Society for Optical Engineering. 2000 ; Vol. 3924. pp. 18-26.
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Manipulation of lipid bilayer membranes in solution using laser tweezers and microsphere 'handles'. / Keating, Christine Dolan; D'Onofrio, Terrence G.; Hatzor, Anat; Whelpley, Amy S.; Natan, Michael J.; Weiss, Paul S.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 3924, 01.01.2000, p. 18-26.

Research output: Contribution to journalConference article

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AU - Keating, Christine Dolan

AU - D'Onofrio, Terrence G.

AU - Hatzor, Anat

AU - Whelpley, Amy S.

AU - Natan, Michael J.

AU - Weiss, Paul S.

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