Mannitol inhibits growth of intact cucumber but not pea seedlings by mechanically collapsing the root pressure

R. Stahlberg, Daniel J. Cosgrove

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The positive xylem pressure (P(x)) in cucumber hypocotyls is a direct extension of root pressure and therefore depends on the root environment. Solutions of the electrolyte KCI (0.10 osm) reduced the hypocotyl P(x) transiently (bipbasic response), while the P(x) reduction by mannitol solutions was sustained. The amplitudes of the induced P(x) reduction depended directly, and the degree of P(x) restoration after stress release depended indirectly, on the size of the initial positive P(x), indicating that mannitol released the root pressure by a mechanical rather than osmotic mechanism. Mannitol treatment and other means of root pressure reduction revealed two separate growth responses in the affected cucumber hypocotyls. Only steep P(x) drops (following root excision or root pressure release in mannitol) directly cause a rapid, transient drop in growth rate (GR). Both rapid and slow (after root incubation in KCN or NEM) decreases in root pressure, however, led to a sustained growth inhibition of cucumber hypocotyls after about 30 min. This delay characterizes the growth response as an indirect consequence of the P(x) change. Pea seedlings, which lacked root pressure and had a negative P(x) throughout, showed extremely small changes in epicotyl P(x) and GR after root incubation in mannitol. It is apparent that the higher sensitivity of cucumber growth to mannitol depended on the presence and release of root pressure.

Original languageEnglish (US)
Pages (from-to)1135-1144
Number of pages10
JournalPlant, Cell and Environment
Volume20
Issue number9
DOIs
StatePublished - Jan 1 1997

Fingerprint

root pressure
Cucumis sativus
Peas
Mannitol
mannitol
Seedlings
cucumbers
xylem
peas
Xylem
Pressure
seedlings
Growth
hypocotyls
Hypocotyl
epicotyls
growth retardation
electrolytes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

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title = "Mannitol inhibits growth of intact cucumber but not pea seedlings by mechanically collapsing the root pressure",
abstract = "The positive xylem pressure (P(x)) in cucumber hypocotyls is a direct extension of root pressure and therefore depends on the root environment. Solutions of the electrolyte KCI (0.10 osm) reduced the hypocotyl P(x) transiently (bipbasic response), while the P(x) reduction by mannitol solutions was sustained. The amplitudes of the induced P(x) reduction depended directly, and the degree of P(x) restoration after stress release depended indirectly, on the size of the initial positive P(x), indicating that mannitol released the root pressure by a mechanical rather than osmotic mechanism. Mannitol treatment and other means of root pressure reduction revealed two separate growth responses in the affected cucumber hypocotyls. Only steep P(x) drops (following root excision or root pressure release in mannitol) directly cause a rapid, transient drop in growth rate (GR). Both rapid and slow (after root incubation in KCN or NEM) decreases in root pressure, however, led to a sustained growth inhibition of cucumber hypocotyls after about 30 min. This delay characterizes the growth response as an indirect consequence of the P(x) change. Pea seedlings, which lacked root pressure and had a negative P(x) throughout, showed extremely small changes in epicotyl P(x) and GR after root incubation in mannitol. It is apparent that the higher sensitivity of cucumber growth to mannitol depended on the presence and release of root pressure.",
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Mannitol inhibits growth of intact cucumber but not pea seedlings by mechanically collapsing the root pressure. / Stahlberg, R.; Cosgrove, Daniel J.

In: Plant, Cell and Environment, Vol. 20, No. 9, 01.01.1997, p. 1135-1144.

Research output: Contribution to journalArticle

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AU - Cosgrove, Daniel J.

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