MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease

John P. Bannantine, Cari K. Lingle, Judith R. Stabel, Kasra X. Ramyar, Brandon L. Garcia, Alex J. Raeber, Pascal Schacher, Vivek Kapur, Brian V. Geisbrecht

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The protein encoded by MAP1272c has been shown to be an antigen of Mycobacterium avium subsp. paratuberculosis that contains an NlpC/P60 superfamily domain found in lipoproteins or integral membrane proteins. Proteins containing this domain have diverse enzymatic functions that include peptidases, amidases, and acetyltransferases. The NlpC protein was examined in comparison to over 100 recombinant proteins and showed the strongest antigenicity when analyzed with sera from cattle with Johne's disease. To further localize the immunogenicity of NlpC, recombinant proteins representing defined regions were expressed and evaluated with sera from cattle with Johne's disease. The region from amino acids 74 to 279 was shown to be the most immunogenic. This fragment was also evaluated against a commercially available enzyme-linked immunosorbent assay (ELISA). Two monoclonal antibodies were produced in mice immunized with the full-length protein, and each recognized a distinct epitope. These antibodies cross-reacted with proteins from other mycobacterial species and demonstrated variable sizes of the proteins expressed from these subspecies. Both antibodies were further analyzed, and their interaction with MAP1272c and MAP1204 was characterized by a solution-based, luminescent binding assay. These tools provide additional means to study a strong antigen of M. avium subsp. paratuberculosis.

Original languageEnglish (US)
Pages (from-to)1083-1092
Number of pages10
JournalClinical and Vaccine Immunology
Volume19
Issue number7
DOIs
StatePublished - Jul 1 2012

Fingerprint

Paratuberculosis
Antigens
Mycobacterium avium
Proteins
Recombinant Proteins
Amidohydrolases
Assays
Luminescent Measurements
Acetyltransferases
Antibodies
Immunosorbents
Serum
Lipoproteins
Epitopes
Membrane Proteins
Peptide Hydrolases
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Amino Acids
Enzymes

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Bannantine, J. P., Lingle, C. K., Stabel, J. R., Ramyar, K. X., Garcia, B. L., Raeber, A. J., ... Geisbrecht, B. V. (2012). MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease. Clinical and Vaccine Immunology, 19(7), 1083-1092. https://doi.org/10.1128/CVI.00195-12
Bannantine, John P. ; Lingle, Cari K. ; Stabel, Judith R. ; Ramyar, Kasra X. ; Garcia, Brandon L. ; Raeber, Alex J. ; Schacher, Pascal ; Kapur, Vivek ; Geisbrecht, Brian V. / MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease. In: Clinical and Vaccine Immunology. 2012 ; Vol. 19, No. 7. pp. 1083-1092.
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Bannantine, JP, Lingle, CK, Stabel, JR, Ramyar, KX, Garcia, BL, Raeber, AJ, Schacher, P, Kapur, V & Geisbrecht, BV 2012, 'MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease', Clinical and Vaccine Immunology, vol. 19, no. 7, pp. 1083-1092. https://doi.org/10.1128/CVI.00195-12

MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease. / Bannantine, John P.; Lingle, Cari K.; Stabel, Judith R.; Ramyar, Kasra X.; Garcia, Brandon L.; Raeber, Alex J.; Schacher, Pascal; Kapur, Vivek; Geisbrecht, Brian V.

In: Clinical and Vaccine Immunology, Vol. 19, No. 7, 01.07.2012, p. 1083-1092.

Research output: Contribution to journalArticle

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T1 - MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease

AU - Bannantine, John P.

AU - Lingle, Cari K.

AU - Stabel, Judith R.

AU - Ramyar, Kasra X.

AU - Garcia, Brandon L.

AU - Raeber, Alex J.

AU - Schacher, Pascal

AU - Kapur, Vivek

AU - Geisbrecht, Brian V.

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Y1 - 2012/7/1

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Bannantine JP, Lingle CK, Stabel JR, Ramyar KX, Garcia BL, Raeber AJ et al. MAP1272c encodes an NlpC/P60 protein, an antigen detected in cattle with Johne's disease. Clinical and Vaccine Immunology. 2012 Jul 1;19(7):1083-1092. https://doi.org/10.1128/CVI.00195-12