Mapping and association of GAD2 and GIP gene variants with feed intake and carcass traits in beef cattle (short communication)

Hong Guo, Wansheng Liu, Akiko Takasuga, Katie Eyer, Earl Landrito, Shang Zhong Xu, Xue Gao, Hong Yan Ren

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Glutamate decarboxylase 2 (GAD2) is a major autoantigen in insulin-dependent diabetes, while glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal hormone. Both genes are involved in insulin secretion and play a role in the regulation of feed intake and metabolic rate in animals. In the present study, we assigned the bovine GAD2 and GIP by RH mapping to BTA 13 and BTA 19, respectively. We discovered SNPs from these genes by a PCR-sequencing approach. We identified one SNP (T/C transition) from the 14th intron of the bovine GAD2 gene and another SNP (A/C transversion) from the 4th intron of the bovine GIP gene. Genotyping over 300 animals from five different beef populations revealed that the average allelic frequency for the GAD2 allele A was 0.48 and allele B 0.52, while for the GIP allele A 0.80 and allele B 0.20, respectively. There were significant associations observed between the GAD2 and GIP gene variants and Average Daily Feed Intake (ADFI) (p<0.05) in beef cattle. In addition, the GAD2 SNP was also associated with Meat Percent (MP) (p<0.05), whereas the GIP SNP was also significantly associated with Backfat Thickness (BF) (p<0.05) and Ratio of Feed-to-Meat (RFM) (p<0.05). There were no significant effects found for other traits. Although both SNPs could be used as candidate markers for MAS to improve feed intake and carcass traits, further investigations in large populations and in other breeds are recommended in order to understand the effect of the GAD2 and GIP polymorphisms on these quantitative traits in beef cattle.

Original languageEnglish (US)
Pages (from-to)33-41
Number of pages9
JournalArchiv fur Tierzucht
Volume51
Issue number1
StatePublished - Mar 13 2008

Fingerprint

gastric inhibitory polypeptide
glutamate decarboxylase
carcass characteristics
beef cattle
animal communication
feed intake
Single Nucleotide Polymorphism
Communication
Glucose
Peptides
Genes
Alleles
genes
alleles
Meat
Introns
introns
cattle
gastrointestinal hormones
meat

All Science Journal Classification (ASJC) codes

  • Food Animals
  • Animal Science and Zoology

Cite this

Guo, Hong ; Liu, Wansheng ; Takasuga, Akiko ; Eyer, Katie ; Landrito, Earl ; Xu, Shang Zhong ; Gao, Xue ; Ren, Hong Yan. / Mapping and association of GAD2 and GIP gene variants with feed intake and carcass traits in beef cattle (short communication). In: Archiv fur Tierzucht. 2008 ; Vol. 51, No. 1. pp. 33-41.
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abstract = "Glutamate decarboxylase 2 (GAD2) is a major autoantigen in insulin-dependent diabetes, while glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal hormone. Both genes are involved in insulin secretion and play a role in the regulation of feed intake and metabolic rate in animals. In the present study, we assigned the bovine GAD2 and GIP by RH mapping to BTA 13 and BTA 19, respectively. We discovered SNPs from these genes by a PCR-sequencing approach. We identified one SNP (T/C transition) from the 14th intron of the bovine GAD2 gene and another SNP (A/C transversion) from the 4th intron of the bovine GIP gene. Genotyping over 300 animals from five different beef populations revealed that the average allelic frequency for the GAD2 allele A was 0.48 and allele B 0.52, while for the GIP allele A 0.80 and allele B 0.20, respectively. There were significant associations observed between the GAD2 and GIP gene variants and Average Daily Feed Intake (ADFI) (p<0.05) in beef cattle. In addition, the GAD2 SNP was also associated with Meat Percent (MP) (p<0.05), whereas the GIP SNP was also significantly associated with Backfat Thickness (BF) (p<0.05) and Ratio of Feed-to-Meat (RFM) (p<0.05). There were no significant effects found for other traits. Although both SNPs could be used as candidate markers for MAS to improve feed intake and carcass traits, further investigations in large populations and in other breeds are recommended in order to understand the effect of the GAD2 and GIP polymorphisms on these quantitative traits in beef cattle.",
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Guo, H, Liu, W, Takasuga, A, Eyer, K, Landrito, E, Xu, SZ, Gao, X & Ren, HY 2008, 'Mapping and association of GAD2 and GIP gene variants with feed intake and carcass traits in beef cattle (short communication)', Archiv fur Tierzucht, vol. 51, no. 1, pp. 33-41.

Mapping and association of GAD2 and GIP gene variants with feed intake and carcass traits in beef cattle (short communication). / Guo, Hong; Liu, Wansheng; Takasuga, Akiko; Eyer, Katie; Landrito, Earl; Xu, Shang Zhong; Gao, Xue; Ren, Hong Yan.

In: Archiv fur Tierzucht, Vol. 51, No. 1, 13.03.2008, p. 33-41.

Research output: Contribution to journalArticle

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AU - Guo, Hong

AU - Liu, Wansheng

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AU - Xu, Shang Zhong

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AU - Ren, Hong Yan

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N2 - Glutamate decarboxylase 2 (GAD2) is a major autoantigen in insulin-dependent diabetes, while glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal hormone. Both genes are involved in insulin secretion and play a role in the regulation of feed intake and metabolic rate in animals. In the present study, we assigned the bovine GAD2 and GIP by RH mapping to BTA 13 and BTA 19, respectively. We discovered SNPs from these genes by a PCR-sequencing approach. We identified one SNP (T/C transition) from the 14th intron of the bovine GAD2 gene and another SNP (A/C transversion) from the 4th intron of the bovine GIP gene. Genotyping over 300 animals from five different beef populations revealed that the average allelic frequency for the GAD2 allele A was 0.48 and allele B 0.52, while for the GIP allele A 0.80 and allele B 0.20, respectively. There were significant associations observed between the GAD2 and GIP gene variants and Average Daily Feed Intake (ADFI) (p<0.05) in beef cattle. In addition, the GAD2 SNP was also associated with Meat Percent (MP) (p<0.05), whereas the GIP SNP was also significantly associated with Backfat Thickness (BF) (p<0.05) and Ratio of Feed-to-Meat (RFM) (p<0.05). There were no significant effects found for other traits. Although both SNPs could be used as candidate markers for MAS to improve feed intake and carcass traits, further investigations in large populations and in other breeds are recommended in order to understand the effect of the GAD2 and GIP polymorphisms on these quantitative traits in beef cattle.

AB - Glutamate decarboxylase 2 (GAD2) is a major autoantigen in insulin-dependent diabetes, while glucose-dependent insulinotropic polypeptide (GIP) is a gastrointestinal hormone. Both genes are involved in insulin secretion and play a role in the regulation of feed intake and metabolic rate in animals. In the present study, we assigned the bovine GAD2 and GIP by RH mapping to BTA 13 and BTA 19, respectively. We discovered SNPs from these genes by a PCR-sequencing approach. We identified one SNP (T/C transition) from the 14th intron of the bovine GAD2 gene and another SNP (A/C transversion) from the 4th intron of the bovine GIP gene. Genotyping over 300 animals from five different beef populations revealed that the average allelic frequency for the GAD2 allele A was 0.48 and allele B 0.52, while for the GIP allele A 0.80 and allele B 0.20, respectively. There were significant associations observed between the GAD2 and GIP gene variants and Average Daily Feed Intake (ADFI) (p<0.05) in beef cattle. In addition, the GAD2 SNP was also associated with Meat Percent (MP) (p<0.05), whereas the GIP SNP was also significantly associated with Backfat Thickness (BF) (p<0.05) and Ratio of Feed-to-Meat (RFM) (p<0.05). There were no significant effects found for other traits. Although both SNPs could be used as candidate markers for MAS to improve feed intake and carcass traits, further investigations in large populations and in other breeds are recommended in order to understand the effect of the GAD2 and GIP polymorphisms on these quantitative traits in beef cattle.

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