Mapping, expression, and association study of the bovine PSMC1 gene

H. Guo, Wansheng Liu, A. Takasuga, K. Eyer, E. Landrito, S. Z. Xu, X. Gao, H. Y. Ren

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The 26S ATP-dependent protease is composed of a 20S catalytic proteasome and two PA700 regulatory modules; it plays a central role in many regulatory pathways, such as cell cycle regulation, differentiation, and apoptosis. The PA700 complex is composed of multiple subunits, including at least six related ATPases and approximately 15 non-ATPase polypeptides. PSMC1 (proteasome 26S subunit, ATPase, 1) is one of these ATPases. In this study, we amplified a fragment of 507 bp from intron 9 of the bovine PSMC1 gene and found a SNP (G/A) at position 216 in the PCR fragment. Genotyping of 138 animals from four beef breeds revealed that the average frequency for allele A (G-base) was 0.4271 (0.3269-0.5517); for allele B (A-base) it was 0.5729 (0.4483-0.6731). This SNP is significantly associated with average daily feed intake (P < 0.01), average daily gain, finishing average daily gain, body length, ratio of feed to meat, backfat thickness, and loin-muscle area (P < 0.05). Our experimental data showed that animals with an AA genotype have a significantly lower food intake, grow faster, are longer in the body, and have less backfat and bigger loin muscle; hence, their ratio of feed to meat is significantly lower. We believe that the PSMC1 SNP is a potential candidate marker for marker-assisted selection in these traits. We also found that the bovine PSMC1 gene was expressed mainly in lung, testis, and spleen. In addition, we mapped the bovine PSMC1 gene on BTA10 by an RH mapping method.

Original languageEnglish (US)
Pages (from-to)347-355
Number of pages9
JournalBiochemical Genetics
Volume46
Issue number5-6
DOIs
StatePublished - Jun 1 2008

Fingerprint

Single Nucleotide Polymorphism
adenosinetriphosphatase
Adenosine Triphosphatases
Meats
Genes
proteasome endopeptidase complex
loins
backfat
average daily gain
meat
Meat
Muscle
gene
cattle
allele
Animals
muscle
Beef
Muscles
muscles

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Guo, H., Liu, W., Takasuga, A., Eyer, K., Landrito, E., Xu, S. Z., ... Ren, H. Y. (2008). Mapping, expression, and association study of the bovine PSMC1 gene. Biochemical Genetics, 46(5-6), 347-355. https://doi.org/10.1007/s10528-008-9151-0
Guo, H. ; Liu, Wansheng ; Takasuga, A. ; Eyer, K. ; Landrito, E. ; Xu, S. Z. ; Gao, X. ; Ren, H. Y. / Mapping, expression, and association study of the bovine PSMC1 gene. In: Biochemical Genetics. 2008 ; Vol. 46, No. 5-6. pp. 347-355.
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Guo, H, Liu, W, Takasuga, A, Eyer, K, Landrito, E, Xu, SZ, Gao, X & Ren, HY 2008, 'Mapping, expression, and association study of the bovine PSMC1 gene', Biochemical Genetics, vol. 46, no. 5-6, pp. 347-355. https://doi.org/10.1007/s10528-008-9151-0

Mapping, expression, and association study of the bovine PSMC1 gene. / Guo, H.; Liu, Wansheng; Takasuga, A.; Eyer, K.; Landrito, E.; Xu, S. Z.; Gao, X.; Ren, H. Y.

In: Biochemical Genetics, Vol. 46, No. 5-6, 01.06.2008, p. 347-355.

Research output: Contribution to journalArticle

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T1 - Mapping, expression, and association study of the bovine PSMC1 gene

AU - Guo, H.

AU - Liu, Wansheng

AU - Takasuga, A.

AU - Eyer, K.

AU - Landrito, E.

AU - Xu, S. Z.

AU - Gao, X.

AU - Ren, H. Y.

PY - 2008/6/1

Y1 - 2008/6/1

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AB - The 26S ATP-dependent protease is composed of a 20S catalytic proteasome and two PA700 regulatory modules; it plays a central role in many regulatory pathways, such as cell cycle regulation, differentiation, and apoptosis. The PA700 complex is composed of multiple subunits, including at least six related ATPases and approximately 15 non-ATPase polypeptides. PSMC1 (proteasome 26S subunit, ATPase, 1) is one of these ATPases. In this study, we amplified a fragment of 507 bp from intron 9 of the bovine PSMC1 gene and found a SNP (G/A) at position 216 in the PCR fragment. Genotyping of 138 animals from four beef breeds revealed that the average frequency for allele A (G-base) was 0.4271 (0.3269-0.5517); for allele B (A-base) it was 0.5729 (0.4483-0.6731). This SNP is significantly associated with average daily feed intake (P < 0.01), average daily gain, finishing average daily gain, body length, ratio of feed to meat, backfat thickness, and loin-muscle area (P < 0.05). Our experimental data showed that animals with an AA genotype have a significantly lower food intake, grow faster, are longer in the body, and have less backfat and bigger loin muscle; hence, their ratio of feed to meat is significantly lower. We believe that the PSMC1 SNP is a potential candidate marker for marker-assisted selection in these traits. We also found that the bovine PSMC1 gene was expressed mainly in lung, testis, and spleen. In addition, we mapped the bovine PSMC1 gene on BTA10 by an RH mapping method.

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