Mapping of candidate tumor suppressor genes on chromosome 12 in adenoid cystic carcinoma

Susan Rutherford Siegel, Garret M. Hampton, Henry F. Frierson, Christopher A. Moskaluk

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Adenoid cystic carcinoma (ACC) is a common malignancy of salivary glands, for which the underlying genetic mechanisms of tumorigenesis are poorly understood. Prior studies in ACC have identified deletions in chromosome 12. To further characterize these changes, we performed an extensive LOH analysis in 58 ACC using a panel of 28 microsatellite markers. Results show 66% overall genetic loss. Three markers (D12S1713, D12S2196, D12S398) are contiguous and define a 6.84 Mb region of deletion at 12q13.11-q13.13. Two other markers (D12S2078, D12S1628) are also contiguous and define a 4.5 Mb region of deletion at 12q24.32-q24.33. The three remaining markers, D12S1056 at 12q14.1, D12S1051 at 12q23.1 and D12S1636 at 12q23.3 define smaller regions of deletion. An analysis of microarray gene expression profiling data available for ACC shows several genes with significant transcriptional downregulation that map to these areas of genetic deletion. This combined genetic and genomic analysis provides several candidate genes to test for functional tumor suppressor activity in ACC.

Original languageEnglish (US)
Pages (from-to)1076-1085
Number of pages10
JournalLaboratory Investigation
Volume85
Issue number9
DOIs
StatePublished - Dec 1 2005

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Chromosomes, Human, Pair 12
Adenoid Cystic Carcinoma
Tumor Suppressor Genes
Gene Expression Profiling
Microarray Analysis
Salivary Glands
Microsatellite Repeats
Genes
Neoplasms
Carcinogenesis
Down-Regulation

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

Cite this

Siegel, Susan Rutherford ; Hampton, Garret M. ; Frierson, Henry F. ; Moskaluk, Christopher A. / Mapping of candidate tumor suppressor genes on chromosome 12 in adenoid cystic carcinoma. In: Laboratory Investigation. 2005 ; Vol. 85, No. 9. pp. 1076-1085.
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Mapping of candidate tumor suppressor genes on chromosome 12 in adenoid cystic carcinoma. / Siegel, Susan Rutherford; Hampton, Garret M.; Frierson, Henry F.; Moskaluk, Christopher A.

In: Laboratory Investigation, Vol. 85, No. 9, 01.12.2005, p. 1076-1085.

Research output: Contribution to journalArticle

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