Mapping of cotton-melon aphid resistance in melon

J. Klingler, I. Kovalski, L. Silberstein, Gary Allen Thompson, R. Perl-Treves

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Resistance to cotton-melon aphid (Aphis gossypii Glover) segregated as a single dominant gene in a melon (Cucumis melo L.) mapping population derived from the cross 'Top Mark' xPI414723. Sixty-four F2-derived F3 families were used to map the aphid resistance locus, Vat, with respect to randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers. RFLP markers NBS-2 and AC-39 flanked Vat at distances of 3.1 cM and 6.4 cM, respectively. NBS-2 is homologous to the nucleotide binding site-leucine-rich repeat (NBS-LRR) superfamily of plant resistance genes. Another homolog of this superfamily, NBS-5, was positioned ≈16.8 cM from Vat, raising the possibility that Vat resides in a cluster of NBS-LRR paralogs. RFLP marker AC-8, which has similarity to plant lipoxygenases, was positioned at ≈5.5 cM from Vat. Monogenic resistance to A. gossypii has been identified in two sources of melon germplasm, Indian accession PI 371795 (progenitor of PI 414723) and Korean accession PI 161375. To test for an allelic relation between the genes controlling aphid resistance in these two distinct germplasm sources, melon plants of a backcross population from a cross between two resistant lines having Indian- or Korean-derived resistance were infested with aphids. At least 90 out of 92 segregating progeny were aphid resistant, suggesting that the same resistance gene, Vat, is present in both sources of melon germplasm.

Original languageEnglish (US)
Pages (from-to)56-63
Number of pages8
JournalJournal of the American Society for Horticultural Science
Volume126
Issue number1
StatePublished - Jan 1 2001

Fingerprint

Cucurbitaceae
Aphids
Aphis gossypii
melons
Aphidoidea
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
germplasm
Leucine
leucine
binding sites
Nucleotides
nucleotides
Binding Sites
Cucumis melo
Lipoxygenases
Dominant Genes
Plant Genes
genes
dominant genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Horticulture

Cite this

Klingler, J., Kovalski, I., Silberstein, L., Thompson, G. A., & Perl-Treves, R. (2001). Mapping of cotton-melon aphid resistance in melon. Journal of the American Society for Horticultural Science, 126(1), 56-63.
Klingler, J. ; Kovalski, I. ; Silberstein, L. ; Thompson, Gary Allen ; Perl-Treves, R. / Mapping of cotton-melon aphid resistance in melon. In: Journal of the American Society for Horticultural Science. 2001 ; Vol. 126, No. 1. pp. 56-63.
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Klingler, J, Kovalski, I, Silberstein, L, Thompson, GA & Perl-Treves, R 2001, 'Mapping of cotton-melon aphid resistance in melon', Journal of the American Society for Horticultural Science, vol. 126, no. 1, pp. 56-63.

Mapping of cotton-melon aphid resistance in melon. / Klingler, J.; Kovalski, I.; Silberstein, L.; Thompson, Gary Allen; Perl-Treves, R.

In: Journal of the American Society for Horticultural Science, Vol. 126, No. 1, 01.01.2001, p. 56-63.

Research output: Contribution to journalArticle

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Klingler J, Kovalski I, Silberstein L, Thompson GA, Perl-Treves R. Mapping of cotton-melon aphid resistance in melon. Journal of the American Society for Horticultural Science. 2001 Jan 1;126(1):56-63.