Mapping viral functional domains for genetic diversity in plants

Justin S. Pita, Marilyn J. Roossinck

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cucumber mosaic virus (CMV) comprises numerous isolates with various levels of in-host diversity. Subgroup-distinctive features of the Fny and LS strains provided us with a platform to genetically map the viral control elements for genetic variation in planta. We found that both RNAs 1 and 2 controlled levels of genetic diversity, and further fine mapping revealed that the control elements of mutation frequency reside within the first 596 amino acids (aa) ofRNA1. The 2a/2b overlapping region of the 2a protein also contributed to control of viral genetic variation. Furthermore, the 3' nontranslated region (NTR) of RNA 3 constituted a hot spot of polymorphism, where the majority of fixed mutations found in the population were clustered. The 2b gene of CMV, a viral suppressor of gene silencing, controls the abundance of the fixed mutants in the viral population via a host-dependent mechanism.

Original languageEnglish (US)
Pages (from-to)790-797
Number of pages8
JournalJournal of virology
Volume87
Issue number2
DOIs
StatePublished - Jan 1 2013

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Cucumovirus
Cucumber mosaic virus
genetic variation
RNA
suppressor genes
Suppressor Genes
mutation
microbial genetics
Untranslated RNA
Viral Genes
Gene Silencing
gene silencing
Mutation Rate
Plantae
Population
genetic polymorphism
Amino Acids
mutants
Mutation
amino acids

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

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Mapping viral functional domains for genetic diversity in plants. / Pita, Justin S.; Roossinck, Marilyn J.

In: Journal of virology, Vol. 87, No. 2, 01.01.2013, p. 790-797.

Research output: Contribution to journalArticle

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