Mass spectrometric assay for analysis of haptoglobin fucosylation in pancreatic cancer

Zhenxin Lin, Diane M. Simeone, Michelle A. Anderson, Randall E. Brand, Xiaolei Xie, Kerby A. Shedden, Mack Ruffin, David M. Lubman

Research output: Contribution to journalArticle

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Abstract

A mass spectrometric method was developed to elucidate the N-glycan structures of serum glycoproteins and utilize fucosylated glycans as potential markers for pancreatic cancer. This assay was applied to haptoglobin in human serum where N-glycans derived from the serum of 16 pancreatic cancer patients were compared with those from 15 individuals with benign conditions (5 normals, 5 chronic pancreatitis, and 5 type II diabetes). This assay used only 10 μL of serum where haptoglobin was extracted using a monoclonal antibody and quantitative permethylation was performed on desialylated N-glycans followed by MALDI-QIT-TOF MS analysis. Eight desialylated N-glycan structures of haptoglobin were identified where a bifucosylated triantennary structure was reported for the first time in pancreatic cancer samples. Both core and antennary fucosylation were elevated in pancreatic cancer samples compared to samples from benign conditions. Fucosylation degree indices were calculated and show a significant difference between pancreatic cancer patients of all stages and the benign conditions analyzed. This study demonstrates that a serum assay based on haptoglobin fucosylation patterns using mass spectrometric analysis may serve as a novel method for the diagnosis of pancreatic cancer.

Original languageEnglish (US)
Pages (from-to)2602-2611
Number of pages10
JournalJournal of Proteome Research
Volume10
Issue number5
DOIs
StatePublished - May 6 2011

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Haptoglobins
Pancreatic Neoplasms
Polysaccharides
Assays
Serum
Medical problems
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Chronic Pancreatitis
Glycoproteins
Type 2 Diabetes Mellitus
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

Cite this

Lin, Z., Simeone, D. M., Anderson, M. A., Brand, R. E., Xie, X., Shedden, K. A., ... Lubman, D. M. (2011). Mass spectrometric assay for analysis of haptoglobin fucosylation in pancreatic cancer. Journal of Proteome Research, 10(5), 2602-2611. https://doi.org/10.1021/pr200102h
Lin, Zhenxin ; Simeone, Diane M. ; Anderson, Michelle A. ; Brand, Randall E. ; Xie, Xiaolei ; Shedden, Kerby A. ; Ruffin, Mack ; Lubman, David M. / Mass spectrometric assay for analysis of haptoglobin fucosylation in pancreatic cancer. In: Journal of Proteome Research. 2011 ; Vol. 10, No. 5. pp. 2602-2611.
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Lin, Z, Simeone, DM, Anderson, MA, Brand, RE, Xie, X, Shedden, KA, Ruffin, M & Lubman, DM 2011, 'Mass spectrometric assay for analysis of haptoglobin fucosylation in pancreatic cancer', Journal of Proteome Research, vol. 10, no. 5, pp. 2602-2611. https://doi.org/10.1021/pr200102h

Mass spectrometric assay for analysis of haptoglobin fucosylation in pancreatic cancer. / Lin, Zhenxin; Simeone, Diane M.; Anderson, Michelle A.; Brand, Randall E.; Xie, Xiaolei; Shedden, Kerby A.; Ruffin, Mack; Lubman, David M.

In: Journal of Proteome Research, Vol. 10, No. 5, 06.05.2011, p. 2602-2611.

Research output: Contribution to journalArticle

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Lin Z, Simeone DM, Anderson MA, Brand RE, Xie X, Shedden KA et al. Mass spectrometric assay for analysis of haptoglobin fucosylation in pancreatic cancer. Journal of Proteome Research. 2011 May 6;10(5):2602-2611. https://doi.org/10.1021/pr200102h