Mass spectrometry imaging of membrane lipids in cryogenically preserved single cells

Thomas P. Roddy, Sara G. Ostrowski, Donald M. Cannon, Andrew G. Ewing, Nicholas Winograd

Research output: Contribution to conferencePaper

Abstract

The secondary ion mass spectrometry (SIMS) imaging of single paramecium and rat pheochromocytoma (PC) cells was described. Images of paramecia were collected using the latest freeze techniques. It was found that fragments of common membrane lipids, including phosphatidylcholine at 184 m/z and phosphatidylethanolamine at 142 m/z were localized to the cells. The images demonstrated the ability of the time of flight SIMS imaging to analyze phospholipids in single cell membranes.

Original languageEnglish (US)
Pages107-108
Number of pages2
StatePublished - Dec 1 2002
EventProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States
Duration: Jun 2 2002Jun 6 2002

Other

OtherProceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
CountryUnited States
CityOrlando, FL
Period6/2/026/6/02

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Membrane Lipids
Secondary ion mass spectrometry
Mass spectrometry
Imaging techniques
Cell membranes
Phosphatidylcholines
Rats
Phospholipids
phosphatidylethanolamine

All Science Journal Classification (ASJC) codes

  • Spectroscopy

Cite this

Roddy, T. P., Ostrowski, S. G., Cannon, D. M., Ewing, A. G., & Winograd, N. (2002). Mass spectrometry imaging of membrane lipids in cryogenically preserved single cells. 107-108. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.
Roddy, Thomas P. ; Ostrowski, Sara G. ; Cannon, Donald M. ; Ewing, Andrew G. ; Winograd, Nicholas. / Mass spectrometry imaging of membrane lipids in cryogenically preserved single cells. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.2 p.
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Roddy, TP, Ostrowski, SG, Cannon, DM, Ewing, AG & Winograd, N 2002, 'Mass spectrometry imaging of membrane lipids in cryogenically preserved single cells', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02 - 6/6/02 pp. 107-108.

Mass spectrometry imaging of membrane lipids in cryogenically preserved single cells. / Roddy, Thomas P.; Ostrowski, Sara G.; Cannon, Donald M.; Ewing, Andrew G.; Winograd, Nicholas.

2002. 107-108 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conferencePaper

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Roddy TP, Ostrowski SG, Cannon DM, Ewing AG, Winograd N. Mass spectrometry imaging of membrane lipids in cryogenically preserved single cells. 2002. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.