Matrix-assisted laser desorption/ionization mass spectrometric analysis of uncomplexed highly sulfated oligosaccharides using ionic liquid matrices

Tatiana Nikolaevna Laremore, Saravanababu Murugesan, Tae Joon Park, Fikri Y. Avci, Dmitri V. Zagorevski, Robert J. Linhardt

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulfated oligosaccharides do not produce any signal. We report that 1-methylimidazolium α-cyano-4-hydroxycinnamate and butylammonium 2,5-dihydroxybenzoate ionic liquid matrices allow the detection of picomole amounts of the sodium salts of a disaccharide, sucrose octasulfate, and an octasulfated pentasaccharide, Arixtra. The experimental results indicate that both analytes undergo some degree of thermal fragmentation with a mass loss corresponding to cleavage of O-SO3Na bonds in the matrix upon laser irradiation, reflecting lability of sulfo groups.

Original languageEnglish (US)
Pages (from-to)1774-1779
Number of pages6
JournalAnalytical Chemistry
Volume78
Issue number6
DOIs
StatePublished - Mar 15 2006

Fingerprint

Ionic Liquids
Oligosaccharides
Ionization
Desorption
Lasers
Disaccharides
Laser beam effects
Salts
Sodium
Hot Temperature
butylammonium 2,5-dihydroxybenzoate
sucrose octasulfate
fondaparinux

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Laremore, Tatiana Nikolaevna ; Murugesan, Saravanababu ; Park, Tae Joon ; Avci, Fikri Y. ; Zagorevski, Dmitri V. ; Linhardt, Robert J. / Matrix-assisted laser desorption/ionization mass spectrometric analysis of uncomplexed highly sulfated oligosaccharides using ionic liquid matrices. In: Analytical Chemistry. 2006 ; Vol. 78, No. 6. pp. 1774-1779.
@article{4f866e35d17145168a2dd3b4a0a3fc2b,
title = "Matrix-assisted laser desorption/ionization mass spectrometric analysis of uncomplexed highly sulfated oligosaccharides using ionic liquid matrices",
abstract = "Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulfated oligosaccharides do not produce any signal. We report that 1-methylimidazolium α-cyano-4-hydroxycinnamate and butylammonium 2,5-dihydroxybenzoate ionic liquid matrices allow the detection of picomole amounts of the sodium salts of a disaccharide, sucrose octasulfate, and an octasulfated pentasaccharide, Arixtra. The experimental results indicate that both analytes undergo some degree of thermal fragmentation with a mass loss corresponding to cleavage of O-SO3Na bonds in the matrix upon laser irradiation, reflecting lability of sulfo groups.",
author = "Laremore, {Tatiana Nikolaevna} and Saravanababu Murugesan and Park, {Tae Joon} and Avci, {Fikri Y.} and Zagorevski, {Dmitri V.} and Linhardt, {Robert J.}",
year = "2006",
month = "3",
day = "15",
doi = "10.1021/ac051121q",
language = "English (US)",
volume = "78",
pages = "1774--1779",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "6",

}

Matrix-assisted laser desorption/ionization mass spectrometric analysis of uncomplexed highly sulfated oligosaccharides using ionic liquid matrices. / Laremore, Tatiana Nikolaevna; Murugesan, Saravanababu; Park, Tae Joon; Avci, Fikri Y.; Zagorevski, Dmitri V.; Linhardt, Robert J.

In: Analytical Chemistry, Vol. 78, No. 6, 15.03.2006, p. 1774-1779.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Matrix-assisted laser desorption/ionization mass spectrometric analysis of uncomplexed highly sulfated oligosaccharides using ionic liquid matrices

AU - Laremore, Tatiana Nikolaevna

AU - Murugesan, Saravanababu

AU - Park, Tae Joon

AU - Avci, Fikri Y.

AU - Zagorevski, Dmitri V.

AU - Linhardt, Robert J.

PY - 2006/3/15

Y1 - 2006/3/15

N2 - Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulfated oligosaccharides do not produce any signal. We report that 1-methylimidazolium α-cyano-4-hydroxycinnamate and butylammonium 2,5-dihydroxybenzoate ionic liquid matrices allow the detection of picomole amounts of the sodium salts of a disaccharide, sucrose octasulfate, and an octasulfated pentasaccharide, Arixtra. The experimental results indicate that both analytes undergo some degree of thermal fragmentation with a mass loss corresponding to cleavage of O-SO3Na bonds in the matrix upon laser irradiation, reflecting lability of sulfo groups.

AB - Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulfated oligosaccharides do not produce any signal. We report that 1-methylimidazolium α-cyano-4-hydroxycinnamate and butylammonium 2,5-dihydroxybenzoate ionic liquid matrices allow the detection of picomole amounts of the sodium salts of a disaccharide, sucrose octasulfate, and an octasulfated pentasaccharide, Arixtra. The experimental results indicate that both analytes undergo some degree of thermal fragmentation with a mass loss corresponding to cleavage of O-SO3Na bonds in the matrix upon laser irradiation, reflecting lability of sulfo groups.

UR - http://www.scopus.com/inward/record.url?scp=33645218878&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645218878&partnerID=8YFLogxK

U2 - 10.1021/ac051121q

DO - 10.1021/ac051121q

M3 - Article

C2 - 16536411

AN - SCOPUS:33645218878

VL - 78

SP - 1774

EP - 1779

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 6

ER -