Matrix-assisted laser desorption/ionization mass spectrometric analysis of uncomplexed highly sulfated oligosaccharides using ionic liquid matrices

Tatiana N. Laremore, Saravanababu Murugesan, Tae Joon Park, Fikri Y. Avci, Dmitri V. Zagorevski, Robert J. Linhardt

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

Direct UV matrix-assisted laser desorption/ionization (MALDI) mass spectrometric analysis of uncomplexed, underivatized, highly sulfated oligosaccharides has been carried out using ionic liquids as matrices. Under conventionally used MALDI time-of-flight experimental conditions, uncomplexed polysulfated oligosaccharides do not produce any signal. We report that 1-methylimidazolium α-cyano-4-hydroxycinnamate and butylammonium 2,5-dihydroxybenzoate ionic liquid matrices allow the detection of picomole amounts of the sodium salts of a disaccharide, sucrose octasulfate, and an octasulfated pentasaccharide, Arixtra. The experimental results indicate that both analytes undergo some degree of thermal fragmentation with a mass loss corresponding to cleavage of O-SO3Na bonds in the matrix upon laser irradiation, reflecting lability of sulfo groups.

Original languageEnglish (US)
Pages (from-to)1774-1779
Number of pages6
JournalAnalytical chemistry
Volume78
Issue number6
DOIs
StatePublished - Mar 15 2006

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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