MB-02 Cells undergo fetal to adult globin gene switching in response to erythropoietin

Susan P. Perrine, Barbara A. Miller, Doris A. Morgan

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

The recently described MB-02 human cell line requires Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) for continuous growth and terminally differentiates into enucleate, hemoglobinized cells in response to erythropoietin. Here, analysis of globin production now demonstrates that uninduced MB-02 cells produce α globin and the fetal globin chains Gγ and Aγ in a ratio of 1:3. Addition of erythropoietin results in de novo synthesis of β globin chains and a marked increase in total Hb/ cell. Thus, the MB-02 cell line partially recapitulates the fetal to adult globin switch that occurs during erythroid and human fetal development and provides a new clonal human erythroid progenitor system for investigating the biochemical and molecular events involved in globin gene switching.

Original languageEnglish (US)
Pages (from-to)857-862
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume164
Issue number2
DOIs
StatePublished - Oct 31 1989

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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