Measurement of glutathione requiring enzymes involved in arachidonic acid cascade of rat basophilic leukemia cells

C. C. Reddy, M. K. Rao, A. M. Mastro, R. W. Egan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rat basophilic leukemia cells have frequently been employed for investigating the pathways of leukotriene biosynthesis, a class of biologically active arachidonic acid metabolites. However, information is lacking on the levels of selenium-dependent glutathione peroxidase (Se-GSH-Px), non-Se-GSH-Px and glutathione S-transferases (GSH-S-Trs), key enzymes involved in fatty acid hydroperoxide metabolism and leukotriene biosynthesis in these cells. Both GSH-S-Trs and non-Se-GSH-Px reactions are catalyzed by the same enzyme. In the present studies, we have measured the enzyme activities of GSH-Px(s) and GSH-S-Trs in the 105,000 x g supernatant fraction of sonified RBL-1 cells. The specific activities for GSH-Px(s) toward H2O2, cumene hydroperoxide, and 15S-hydroperoxy-eicosatetraenoic acid (15S-HPETE) are 12.6, 17.9 and 26.9 nmoles.min-1.mg-1 protein, respectively. A specific activity of 18.9 moles.min-1.mg-1 protein with 1-chloro-2,4-dinitrobenzene was estimated for the GSH-S-Trs. Therefore, the cell fraction that exhibits 5-lipoxygenase activity also contains selenium and non-selenium glutathione peroxidases.

Original languageEnglish (US)
Pages (from-to)755-761
Number of pages7
JournalBiochemistry International
Volume9
Issue number6
StatePublished - 1984

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Glutathione Transferase
Arachidonic Acid
Glutathione
Rats
Leukemia
Leukotrienes
Biosynthesis
Enzymes
Selenium
Glutathione Peroxidase
Arachidonic Acids
Dinitrochlorobenzene
Arachidonate 5-Lipoxygenase
Lipid Peroxides
Enzyme activity
Metabolites
Metabolism
Proteins

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Reddy, C. C. ; Rao, M. K. ; Mastro, A. M. ; Egan, R. W. / Measurement of glutathione requiring enzymes involved in arachidonic acid cascade of rat basophilic leukemia cells. In: Biochemistry International. 1984 ; Vol. 9, No. 6. pp. 755-761.
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Measurement of glutathione requiring enzymes involved in arachidonic acid cascade of rat basophilic leukemia cells. / Reddy, C. C.; Rao, M. K.; Mastro, A. M.; Egan, R. W.

In: Biochemistry International, Vol. 9, No. 6, 1984, p. 755-761.

Research output: Contribution to journalArticle

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AU - Egan, R. W.

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