Measurement of Internuclear Distances in Solid-State NMR by a Background-Filtered REDOR Experiment

Frederick G. Vogt, Susan M. Mattingly, James M. Gibson, Karl T. Mueller

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5 Scopus citations

Abstract

A background-filtered version of the rotational-echo double resonance (REDOR) experiment is demonstrated. The experiment combines a traditional REDOR pulse sequence with a double-cross-polarization (DCP) sequence to select only those signals coming from spin pairs of interest. The relatively inefficient DCP sequence, which transfers polarization from 1H to 15N and subsequently to 13C, is improved by the use of adiabatic passages through the (-1) sideband of the Hartmann-Hahn matching condition. The result is an efficient 2D-REDOR pulse sequence that does not require a reference experiment for removal of background signals. The data produced by the experiment are ideally suited to analysis by newly developed dipolar transform methods, such as the REDOR transform. The relevant features of the experiment are demonstrated on simple labeled amino acids. Relative efficiencies of several other potential filtering methods are also compared.

Original languageEnglish (US)
Pages (from-to)26-35
Number of pages10
JournalJournal of Magnetic Resonance
Volume147
Issue number1
DOIs
StatePublished - Nov 2000

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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