Measurement of nitric oxide synthase activity in sections of rat liver

S. Mehdizadeh, A. O'Farrell, L. Bitensky, Judith Weisz, J. Alaghband-Zadeh, J. Chayen

Research output: Contribution to journalArticle

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Abstract

In the previous communication we described a histochemical method for measuring soluble guanylate cyclase (sGC) activity in sections of rat liver. In theory, this method could be used to assess nitric oxide synthase (NOS) activity by the increased sGC activity induced by the additional presence of the substrates for NOS activity. We found that this was correct provided that the concentration of the colloid stabilizer in the reaction medium was decreased to just below the concentration required to fully stabilize the guanylate cyclase activity in the sections. This was related to the fact that the site of NOS activity was different from that of the sGC activity in the hepatocytes, so that the NO generated had to diffuse from the Kupffer cells to the hepatocytes as could occur only in partially unstabilized sections. Optimal concentrations of arginine and of NADPH have been determined for demonstrating NOS activity; the increased reaction was shown to be largely inhibited by methyl-arginine.

Original languageEnglish (US)
Pages (from-to)1229-1234
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume43
Issue number12
StatePublished - Jan 1 1995

Fingerprint

Nitric Oxide Synthase
Liver
Arginine
Hepatocytes
Kupffer Cells
Guanylate Cyclase
Colloids
NADP
Communication
Soluble Guanylyl Cyclase

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

Mehdizadeh, S., O'Farrell, A., Bitensky, L., Weisz, J., Alaghband-Zadeh, J., & Chayen, J. (1995). Measurement of nitric oxide synthase activity in sections of rat liver. Journal of Histochemistry and Cytochemistry, 43(12), 1229-1234.
Mehdizadeh, S. ; O'Farrell, A. ; Bitensky, L. ; Weisz, Judith ; Alaghband-Zadeh, J. ; Chayen, J. / Measurement of nitric oxide synthase activity in sections of rat liver. In: Journal of Histochemistry and Cytochemistry. 1995 ; Vol. 43, No. 12. pp. 1229-1234.
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Mehdizadeh, S, O'Farrell, A, Bitensky, L, Weisz, J, Alaghband-Zadeh, J & Chayen, J 1995, 'Measurement of nitric oxide synthase activity in sections of rat liver', Journal of Histochemistry and Cytochemistry, vol. 43, no. 12, pp. 1229-1234.

Measurement of nitric oxide synthase activity in sections of rat liver. / Mehdizadeh, S.; O'Farrell, A.; Bitensky, L.; Weisz, Judith; Alaghband-Zadeh, J.; Chayen, J.

In: Journal of Histochemistry and Cytochemistry, Vol. 43, No. 12, 01.01.1995, p. 1229-1234.

Research output: Contribution to journalArticle

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Mehdizadeh S, O'Farrell A, Bitensky L, Weisz J, Alaghband-Zadeh J, Chayen J. Measurement of nitric oxide synthase activity in sections of rat liver. Journal of Histochemistry and Cytochemistry. 1995 Jan 1;43(12):1229-1234.