Measuring in vitro extensibility of growing plant cell walls.

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

This article summarizes the theory and practical aspects of measuring cell wall properties by four different extensometer techniques and how the results of these methods relate to the concept and ideal measurement of cell wall extensibility in the context of cell growth. These in vivo techniques are particularly useful for studies of the molecular basis of cell wall extension. Measurements of breaking strength, elastic compliance, and plastic compliance may be informative about changes in cell wall structure, whereas measurements of wall stress relaxation and creep are sensitive to both changes in wall structure and wall-loosening processes, such as those mediated by expansins and some lytic enzymes. A combination of methods is needed to obtain a broader view of cell wall behavior and properties connected with the concept of cell wall extensibility.

Original languageEnglish (US)
Pages (from-to)291-303
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume715
DOIs
StatePublished - Jun 3 2011

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Fingerprint Dive into the research topics of 'Measuring in vitro extensibility of growing plant cell walls.'. Together they form a unique fingerprint.

  • Cite this