Measuring kinetics of synaptic current

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Obtaining an accurate measurement of receptor kinetics is best done using single-channel recordings. However, single channel recordings require activation of channels directly under the patch pipette using either cell-attached patches or inside-out patches, which is difficult when sampling synaptic receptors. In addition, single-channel recordings require receptor activation via exogenously applied agonists. Consequently, receptor activation is elicited by exogenous agonists instead of endogenous neurotransmitters. In order to determine the kinetics of synaptic receptors under physiological environment, the synaptic current can be isolated using the methods described in Chap. 8 and the kinetics can be measured using the approaches described in this chapter.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages121-131
Number of pages11
DOIs
StatePublished - Oct 1 2016

Publication series

NameNeuromethods
Volume112
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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