Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick

Sarah J. Vella, Patrick Beattie, Rebecca Cademartiri, Anna Laromaine, Andres W. Martinez, Scott T. Phillips, Katherine A. Mirica, George M. Whitesides

Research output: Contribution to journalArticlepeer-review

244 Scopus citations

Abstract

This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and "spiked" standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.

Original languageEnglish (US)
Pages (from-to)2883-2891
Number of pages9
JournalAnalytical chemistry
Volume84
Issue number6
DOIs
StatePublished - Mar 20 2012

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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