Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick

Sarah J. Vella, Patrick Beattie, Rebecca Cademartiri, Anna Laromaine, Andres W. Martinez, Scott T. Phillips, Katherine A. Mirica, George M. Whitesides

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and "spiked" standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.

Original languageEnglish (US)
Pages (from-to)2883-2891
Number of pages9
JournalAnalytical Chemistry
Volume84
Issue number6
DOIs
StatePublished - Mar 20 2012

Fingerprint

Plastic sheets
Microfluidics
Liver
Assays
Blood
Aspartate Aminotransferases
Alkaline Phosphatase
Blood Proteins
Calibration
Membranes
Plasmas
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Vella, S. J., Beattie, P., Cademartiri, R., Laromaine, A., Martinez, A. W., Phillips, S. T., ... Whitesides, G. M. (2012). Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick. Analytical Chemistry, 84(6), 2883-2891. https://doi.org/10.1021/ac203434x
Vella, Sarah J. ; Beattie, Patrick ; Cademartiri, Rebecca ; Laromaine, Anna ; Martinez, Andres W. ; Phillips, Scott T. ; Mirica, Katherine A. ; Whitesides, George M. / Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick. In: Analytical Chemistry. 2012 ; Vol. 84, No. 6. pp. 2883-2891.
@article{59a2bb3079b4472e8dce475120ae5bd4,
title = "Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick",
abstract = "This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and {"}spiked{"} standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.",
author = "Vella, {Sarah J.} and Patrick Beattie and Rebecca Cademartiri and Anna Laromaine and Martinez, {Andres W.} and Phillips, {Scott T.} and Mirica, {Katherine A.} and Whitesides, {George M.}",
year = "2012",
month = "3",
day = "20",
doi = "10.1021/ac203434x",
language = "English (US)",
volume = "84",
pages = "2883--2891",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "6",

}

Vella, SJ, Beattie, P, Cademartiri, R, Laromaine, A, Martinez, AW, Phillips, ST, Mirica, KA & Whitesides, GM 2012, 'Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick', Analytical Chemistry, vol. 84, no. 6, pp. 2883-2891. https://doi.org/10.1021/ac203434x

Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick. / Vella, Sarah J.; Beattie, Patrick; Cademartiri, Rebecca; Laromaine, Anna; Martinez, Andres W.; Phillips, Scott T.; Mirica, Katherine A.; Whitesides, George M.

In: Analytical Chemistry, Vol. 84, No. 6, 20.03.2012, p. 2883-2891.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick

AU - Vella, Sarah J.

AU - Beattie, Patrick

AU - Cademartiri, Rebecca

AU - Laromaine, Anna

AU - Martinez, Andres W.

AU - Phillips, Scott T.

AU - Mirica, Katherine A.

AU - Whitesides, George M.

PY - 2012/3/20

Y1 - 2012/3/20

N2 - This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and "spiked" standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.

AB - This paper describes a paper-based microfluidic device that measures two enzymatic markers of liver function (alkaline phosphatase, ALP, and aspartate aminotransferase, AST) and total serum protein. A device consists of four components: (i) a top plastic sheet, (ii) a filter membrane, (iii) a patterned paper chip containing the reagents necessary for analysis, and (iv) a bottom plastic sheet. The device performs both the sample preparation (separating blood plasma from erythrocytes) and the assays; it also enables both qualitative and quantitative analysis of data. The data obtained from the paper-microfluidic devices show standard deviations in calibration runs and "spiked" standards that are acceptable for routine clinical use. This device illustrates a type of test useable for a range of assays in resource-poor settings.

UR - http://www.scopus.com/inward/record.url?scp=84858725997&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84858725997&partnerID=8YFLogxK

U2 - 10.1021/ac203434x

DO - 10.1021/ac203434x

M3 - Article

VL - 84

SP - 2883

EP - 2891

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 6

ER -

Vella SJ, Beattie P, Cademartiri R, Laromaine A, Martinez AW, Phillips ST et al. Measuring markers of liver function using a micropatterned paper device designed for blood from a fingerstick. Analytical Chemistry. 2012 Mar 20;84(6):2883-2891. https://doi.org/10.1021/ac203434x