Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics

Carsten Grashoff, Brenton D. Hoffman, Michael D. Brenner, Ruobo Zhou, Maddy Parsons, Michael T. Yang, Mark A. McLean, Stephen G. Sligar, Christopher S. Chen, Taekjip Ha, Martin A. Schwartz

Research output: Contribution to journalArticlepeer-review

Abstract

Mechanical forces are central to developmental, physiological and pathological processes1. However, limited understanding of force transmission within sub-cellular structures is a major obstacle to unravelling molecular mechanisms. Here we describe the development of a calibrated biosensor that measures forces across specific proteins in cells with piconewton (pN) sensitivity, as demonstrated by single molecule fluorescence force spectroscopy2. The method is applied to vinculin, a protein that connects integrins to actin filaments and whose recruitment to focal adhesions (FAs) is forcedependent3. We show that tension across vinculin in stable FAs is ∼2.5pN and that vinculin recruitment to FAs and force transmission across vinculin are regulated separately. Highest tension across vinculin is associated with adhesion assembly and enlargement. Conversely, vinculin is under low force in disassembling or sliding FAs at the trailing edge of migrating cells. Furthermore, vinculin is required for stabilizing adhesions under force. Together, these data reveal that FA stabilization under force requires both vinculin recruitment and force transmission, and that, surprisingly, these processes can be controlled independently.

Original languageEnglish (US)
Pages (from-to)263-266
Number of pages4
JournalNature
Volume466
Issue number7303
DOIs
StatePublished - Jul 8 2010

All Science Journal Classification (ASJC) codes

  • General

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