Measuring the biomechanical loosening action of bacterial expansins on paper and plant cell walls

Daniel J. Cosgrove, Nathan K. Hepler, Edward R. Wagner, Daniel Michael Durachko

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here, we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages157-165
Number of pages9
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1588
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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    Cosgrove, D. J., Hepler, N. K., Wagner, E. R., & Durachko, D. M. (2017). Measuring the biomechanical loosening action of bacterial expansins on paper and plant cell walls. In Methods in Molecular Biology (pp. 157-165). (Methods in Molecular Biology; Vol. 1588). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6899-2_12