Measuring the folding transition time of single RNA molecules

Tae Hee Lee, Lisa J. Lapidus, Wei Zhao, Kevin J. Travers, Daniel Herschlag, Steven Chu

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We describe a new, time-apertured photon correlation method for resolving the transition time between two states of RNA in folding - i.e., the time of the transition between states rather than the time spent in each state. Single molecule fluorescence resonance energy transfer and fluorescence correlation spectroscopy are used to obtain these measurements. Individual RNA molecules are labeled with fluorophores such as Cy3 and Cy5. Those molecules are then immobilized on a surface and observed for many seconds during which time the molecules spontaneously switch between two conformational states with different levels of flourescence resonance energy transfer efficiency. Single photons are counted from each fluorophore and cross correlated in a small window around a transition. The average of over 1000 cross correlations can be fit to a polynomial, which can determine transition times as short as the average photon emission interval. We applied the method to the P4-P6 domain of the Tetrahymena group I self-splicing intron to yield the folding transition time of 240 μs. The unfolding time is found to be too short to measure with this method.

Original languageEnglish (US)
Pages (from-to)3275-3283
Number of pages9
JournalBiophysical journal
Volume92
Issue number9
DOIs
StatePublished - May 2007

All Science Journal Classification (ASJC) codes

  • Biophysics

Fingerprint Dive into the research topics of 'Measuring the folding transition time of single RNA molecules'. Together they form a unique fingerprint.

  • Cite this

    Lee, T. H., Lapidus, L. J., Zhao, W., Travers, K. J., Herschlag, D., & Chu, S. (2007). Measuring the folding transition time of single RNA molecules. Biophysical journal, 92(9), 3275-3283. https://doi.org/10.1529/biophysj.106.094623