Mechanical Properties of Tandem-Repeat Proteins Are Governed by Network Defects

Abdon Pena-Francesch, Huihun Jung, Mo Segad, Ralph H. Colby, Benjamin D. Allen, Melik C. Demirel

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Topological defects in highly repetitive structural proteins strongly affect their mechanical properties. However, there are no universal rules for structure-property prediction in structural proteins due to high diversity in their repetitive modules. Here, we studied the mechanical properties of tandem-repeat proteins inspired by squid ring teeth proteins using rheology and tensile experiments as well as spectroscopic and X-ray techniques. We also developed a network model based on entropic elasticity to predict structure-property relationships for these proteins. We demonstrated that shear modulus, elastic modulus, and toughness scale inversely with the number of repeats in these proteins. Through optimization of structural repeats, we obtained highly efficient protein network topologies with 42 MJ/m3 ultimate toughness that are capable of withstanding deformations up to 350% when hydrated. Investigation of topological network defects in structural proteins will improve the prediction of mechanical properties for designing novel protein-based materials.

Original languageEnglish (US)
Pages (from-to)884-891
Number of pages8
JournalACS Biomaterials Science and Engineering
Volume4
Issue number3
DOIs
StatePublished - Mar 12 2018

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Tandem Repeat Sequences
Proteins
Mechanical properties
Defects
Toughness
Elastic moduli
Rheology
Elasticity
Topology
X rays

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

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abstract = "Topological defects in highly repetitive structural proteins strongly affect their mechanical properties. However, there are no universal rules for structure-property prediction in structural proteins due to high diversity in their repetitive modules. Here, we studied the mechanical properties of tandem-repeat proteins inspired by squid ring teeth proteins using rheology and tensile experiments as well as spectroscopic and X-ray techniques. We also developed a network model based on entropic elasticity to predict structure-property relationships for these proteins. We demonstrated that shear modulus, elastic modulus, and toughness scale inversely with the number of repeats in these proteins. Through optimization of structural repeats, we obtained highly efficient protein network topologies with 42 MJ/m3 ultimate toughness that are capable of withstanding deformations up to 350{\%} when hydrated. Investigation of topological network defects in structural proteins will improve the prediction of mechanical properties for designing novel protein-based materials.",
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Mechanical Properties of Tandem-Repeat Proteins Are Governed by Network Defects. / Pena-Francesch, Abdon; Jung, Huihun; Segad, Mo; Colby, Ralph H.; Allen, Benjamin D.; Demirel, Melik C.

In: ACS Biomaterials Science and Engineering, Vol. 4, No. 3, 12.03.2018, p. 884-891.

Research output: Contribution to journalArticle

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AU - Pena-Francesch, Abdon

AU - Jung, Huihun

AU - Segad, Mo

AU - Colby, Ralph H.

AU - Allen, Benjamin D.

AU - Demirel, Melik C.

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