Mechanical response of single biological cells to externally applied forces

B. A. Samuel, Md Amanul Haque

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present an experimental technique capable of applying and measuring very small forces (nanoNewtons) on live biological cells. The technique is based on combing microfabricated molded elastomeric microneedles, and substrate deformation to actively apply loads on single cells. By using fluorescence and differential interference contrast imaging we can quantitatively study the response of biological cells to force exerted on it via the extra cellular matrix. Bovine Aortic Endothelial Cells were cultured on the micropillars and strained in-vitro to demonstrate the experimental technique.

Original languageEnglish (US)
Title of host publicationProceedings of the ASME International Mechanical Engineering Congress and Exposition, IMECE 2007
Pages1089-1092
Number of pages4
Volume10 PART B
DOIs
StatePublished - May 30 2008
EventASME International Mechanical Engineering Congress and Exposition, IMECE 2007 - Seattle, WA, United States
Duration: Nov 11 2007Nov 15 2007

Other

OtherASME International Mechanical Engineering Congress and Exposition, IMECE 2007
CountryUnited States
CitySeattle, WA
Period11/11/0711/15/07

Fingerprint

Endothelial cells
Fluorescence
Imaging techniques
Substrates

All Science Journal Classification (ASJC) codes

  • Engineering(all)
  • Mechanical Engineering

Cite this

Samuel, B. A., & Haque, M. A. (2008). Mechanical response of single biological cells to externally applied forces. In Proceedings of the ASME International Mechanical Engineering Congress and Exposition, IMECE 2007 (Vol. 10 PART B, pp. 1089-1092) https://doi.org/10.1115/IMECE2007-43373
Samuel, B. A. ; Haque, Md Amanul. / Mechanical response of single biological cells to externally applied forces. Proceedings of the ASME International Mechanical Engineering Congress and Exposition, IMECE 2007. Vol. 10 PART B 2008. pp. 1089-1092
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Samuel, BA & Haque, MA 2008, Mechanical response of single biological cells to externally applied forces. in Proceedings of the ASME International Mechanical Engineering Congress and Exposition, IMECE 2007. vol. 10 PART B, pp. 1089-1092, ASME International Mechanical Engineering Congress and Exposition, IMECE 2007, Seattle, WA, United States, 11/11/07. https://doi.org/10.1115/IMECE2007-43373

Mechanical response of single biological cells to externally applied forces. / Samuel, B. A.; Haque, Md Amanul.

Proceedings of the ASME International Mechanical Engineering Congress and Exposition, IMECE 2007. Vol. 10 PART B 2008. p. 1089-1092.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Samuel BA, Haque MA. Mechanical response of single biological cells to externally applied forces. In Proceedings of the ASME International Mechanical Engineering Congress and Exposition, IMECE 2007. Vol. 10 PART B. 2008. p. 1089-1092 https://doi.org/10.1115/IMECE2007-43373