Mechanically stimulated osteocytes regulate osteoblastic activity via gap junctions

A. F. Taylor, M. M. Saunders, D. L. Shingle, J. M. Cimbala, Z. Zhou, H. J. Donahue

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

The strong correlation between a bone's architectural properties and the mechanical forces that it experiences has long been attributed to the existence of a cell that not only detects mechanical load but also structurally adapts the bone matrix to counter it. One of the most likely cellular candidates for such a "mechanostat" is the osteocyte, which resides within the mineralized bone matrix and is perfectly situated to detect mechanically induced signals. However, as osteocytes can neither form nor resorb bone, it has been hypothesized that they orchestrate mechanically induced bone remodeling by coordinating the actions of cells residing on the bone surface, such as osteoblasts. To investigate this hypothesis, we developed a novel osteocyte-osteoblast coculture model that mimics in vivo systems by permitting us to expose osteocytes to physiological levels of fluid shear while shielding osteoblasts from it. Our results show that osteocytes exposed to a fluid shear rate of 4.4 dyn/cm2 rapidly increase the alkaline phosphatase activity of the shielded osteoblasts and that osteocytic-osteoblastic physical contact is a prerequisite. Furthermore, both functional gap junctional intercellular communication and the mitogen-activated protein kinase, extracellular signal-regulated kinase 1/2 signaling pathway are essential components in the osteoblastic response to osteocyte communicated mechanical signals. By utilizing other nonosteocytic coculture models, we also show that the ability to mediate osteoblastic alkaline phosphatase levels in response to the application of fluid shear is a phenomena unique to osteocytes and is not reproduced by other mesenchymal cell types.

Original languageEnglish (US)
Pages (from-to)C545-C552
JournalAmerican Journal of Physiology - Cell Physiology
Volume292
Issue number1
DOIs
StatePublished - Jan 1 2007

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Osteocytes
Gap Junctions
Osteoblasts
Bone Matrix
Coculture Techniques
Bone and Bones
Alkaline Phosphatase
Mitogen-Activated Protein Kinase 3
Bone Remodeling
Mitogen-Activated Protein Kinase 1
Mitogen-Activated Protein Kinases

All Science Journal Classification (ASJC) codes

  • Physiology
  • Cell Biology

Cite this

Taylor, A. F. ; Saunders, M. M. ; Shingle, D. L. ; Cimbala, J. M. ; Zhou, Z. ; Donahue, H. J. / Mechanically stimulated osteocytes regulate osteoblastic activity via gap junctions. In: American Journal of Physiology - Cell Physiology. 2007 ; Vol. 292, No. 1. pp. C545-C552.
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Mechanically stimulated osteocytes regulate osteoblastic activity via gap junctions. / Taylor, A. F.; Saunders, M. M.; Shingle, D. L.; Cimbala, J. M.; Zhou, Z.; Donahue, H. J.

In: American Journal of Physiology - Cell Physiology, Vol. 292, No. 1, 01.01.2007, p. C545-C552.

Research output: Contribution to journalArticle

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