Mechanically tunable, human mesenchymal stem cell viable poly(ethylene glycol)–oxime hydrogels with invariant precursor composition, concentration, and stoichiometry

R. A. Dilla, C. M.M. Motta, Y. Xu, Z. K. Zander, N. Bernard, C. G. Wiener, Bryan Vogt, M. L. Becker

Research output: Contribution to journalArticle

Abstract

Hydrogels are used widely for exploratory tissue engineering studies. However, currently, no hydrogel systems have been reported which exhibit a wide range of elastic modulus without changing precursor concentration, identity, or stoichiometry. Herein, ester- and amide-based poly(ethylene glycol) (PEG)–oxime hydrogels with tunable moduli (∼5–30 kPa) were synthesized with identical precursor mass fraction, stoichiometry, and concentration by varying the pH and buffer concentration of the gelation solution, exploiting the kinetics of oxime bond formation. The observed modulus range can be attributed to increasing amounts of network defects in slower forming gels, as confirmed by equilibrium swelling and small-angle neutron scattering (SANS) experiments. Finally, human mesenchymal stem cell (hMSC) viability was confirmed in these materials in a 24-h assay. While this was only an initial demonstration of the potential utility, the controlled variation in defect density and modulus is an important step forward in isolating system variables for hypothesis-driven biological investigations.

Original languageEnglish (US)
Pages (from-to)244-252
Number of pages9
JournalMaterials Today Chemistry
Volume11
DOIs
StatePublished - Mar 1 2019

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Hydrogels
Oximes
Stem cells
Stoichiometry
Polyethylene glycols
Defect density
Hydrogel
Gelation
Neutron scattering
Chemical analysis
Tissue engineering
Amides
Swelling
Assays
Buffers
Esters
Demonstrations
Elastic moduli
Gels
Defects

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Polymers and Plastics
  • Colloid and Surface Chemistry
  • Materials Chemistry

Cite this

Dilla, R. A. ; Motta, C. M.M. ; Xu, Y. ; Zander, Z. K. ; Bernard, N. ; Wiener, C. G. ; Vogt, Bryan ; Becker, M. L. / Mechanically tunable, human mesenchymal stem cell viable poly(ethylene glycol)–oxime hydrogels with invariant precursor composition, concentration, and stoichiometry. In: Materials Today Chemistry. 2019 ; Vol. 11. pp. 244-252.
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Mechanically tunable, human mesenchymal stem cell viable poly(ethylene glycol)–oxime hydrogels with invariant precursor composition, concentration, and stoichiometry. / Dilla, R. A.; Motta, C. M.M.; Xu, Y.; Zander, Z. K.; Bernard, N.; Wiener, C. G.; Vogt, Bryan; Becker, M. L.

In: Materials Today Chemistry, Vol. 11, 01.03.2019, p. 244-252.

Research output: Contribution to journalArticle

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