Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates

Katherine M. Davis, Amie Kathleen Boal

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

X-ray crystallographic characterization of enzymes at different stages in their reaction cycles can provide unique insight into the reaction pathway, the number and type of intermediates formed, and their structural context. The known mechanistic diversity in the radical S-adenosylmethionine (SAM) superfamily of enzymes makes it an appealing target for such studies as more than 100,000 sequences have been identified to date with wide-ranging reactivities that share a pattern of complex radical-mediated chemistry. Here, we review selected examples of radical SAM enzyme crystal structures representative of reactant, product, and intermediate state complexes with a particular emphasis on the strategies employed to capture these states. Broader application of structural characterization techniques to analyze mechanism and substrate specificity is certain to play an important role as more members of this family become tractable for biochemical study.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages331-359
Number of pages29
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Enzymology
Volume595
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Fingerprint

S-Adenosylmethionine
Reaction intermediates
Enzymes
Substrate Specificity
Crystal structure
X-Rays
X rays
Substrates

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Davis, K. M., & Boal, A. K. (2017). Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates. In Methods in Enzymology (pp. 331-359). (Methods in Enzymology; Vol. 595). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2017.07.008
Davis, Katherine M. ; Boal, Amie Kathleen. / Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates. Methods in Enzymology. Academic Press Inc., 2017. pp. 331-359 (Methods in Enzymology).
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Davis, KM & Boal, AK 2017, Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates. in Methods in Enzymology. Methods in Enzymology, vol. 595, Academic Press Inc., pp. 331-359. https://doi.org/10.1016/bs.mie.2017.07.008

Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates. / Davis, Katherine M.; Boal, Amie Kathleen.

Methods in Enzymology. Academic Press Inc., 2017. p. 331-359 (Methods in Enzymology; Vol. 595).

Research output: Chapter in Book/Report/Conference proceedingChapter

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Davis KM, Boal AK. Mechanism-Based Strategies for Structural Characterization of Radical SAM Reaction Intermediates. In Methods in Enzymology. Academic Press Inc. 2017. p. 331-359. (Methods in Enzymology). https://doi.org/10.1016/bs.mie.2017.07.008